RACK1 Interacts with E1A and Rescues E1A-induced Yeast Growth Inhibition and Mammalian Cell Apoptosis Export

@article{citeulike:3736411, abstract = {The adenoviral E1A proteins are able to promote proliferation and transformation, inhibit differentiation, induce apoptosis, and suppress tumor growth. The extreme N terminus and conserved region one of E1A, which are indispensable for transcriptional regulation and for binding to p300/CBP, TBP, and pCAF, play essential roles in these abilities. These observations strongly suggest an intrinsic link between E1A-mediated transcriptional regulation and other effects. In this report, we show that E1A inhibits the normal growth of Saccharomyces cerevisiae HF7c, and this inhibition also depends on the domains required for transcriptional regulation. We demonstrate that E1A associates with histone acetyltransferase activity and represses the transactivation activity of transcription factor in S. cerevisiae, suggesting that E1A may suppress the expression of genes required for normal growth. Based on yeast growth rescue, we present a genetic screening strategy that identified RACK1 as an E1A antagonizing factor. Expression of human RACK1 efficiently relieves E1A-mediated growth inhibition in HF7c and protects human tumor cells from E1A-induced apoptosis. Finally, we show that RACK1 decreases E1A-associated histone acetyltransferase activity in yeast and mammalian cells, and physically interacts with E1A. Our data demonstrate that RACK1 is a repressor of E1A, possibly by antagonizing the effects of E1A on host gene transcription. 10.1074/jbc.M010346200}, author = {Sang, Nianli and Severino, Anna and Russo, Patrizia and Baldi, Alfonso and Giordano, Antonio and Mileo, Anna M. and Paggi, Marco G. and De Luca, Antonio}, citeulike-article-id = {3736411}, citeulike-linkout-0 = {http://dx.doi.org/10.1074/jbc.M010346200}, citeulike-linkout-1 = {http://www.jbc.org/cgi/content/abstract/276/29/27026}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/11358958}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=11358958}, day = {13}, doi = {10.1074/jbc.M010346200}, journal = {J. Biol. Chem.}, keywords = {mammalia, rack1, yeast}, month = {July}, number = {29}, pages = {27026--27033}, posted-at = {2008-12-02 16:42:38}, priority = {2}, title = {RACK1 Interacts with E1A and Rescues E1A-induced Yeast Growth Inhibition and Mammalian Cell Apoptosis}, url = {http://dx.doi.org/10.1074/jbc.M010346200}, volume = {276}, year = {2001} }

TY - JOUR ID - citeulike:3736411 L3 - citeulike-article-id:3736411 N2 - The adenoviral E1A proteins are able to promote proliferation and transformation, inhibit differentiation, induce apoptosis, and suppress tumor growth. The extreme N terminus and conserved region one of E1A, which are indispensable for transcriptional regulation and for binding to p300/CBP, TBP, and pCAF, play essential roles in these abilities. These observations strongly suggest an intrinsic link between E1A-mediated transcriptional regulation and other effects. In this report, we show that E1A inhibits the normal growth of Saccharomyces cerevisiae HF7c, and this inhibition also depends on the domains required for transcriptional regulation. We demonstrate that E1A associates with histone acetyltransferase activity and represses the transactivation activity of transcription factor in S. cerevisiae, suggesting that E1A may suppress the expression of genes required for normal growth. Based on yeast growth rescue, we present a genetic screening strategy that identified RACK1 as an E1A antagonizing factor. Expression of human RACK1 efficiently relieves E1A-mediated growth inhibition in HF7c and protects human tumor cells from E1A-induced apoptosis. Finally, we show that RACK1 decreases E1A-associated histone acetyltransferase activity in yeast and mammalian cells, and physically interacts with E1A. Our data demonstrate that RACK1 is a repressor of E1A, possibly by antagonizing the effects of E1A on host gene transcription. 10.1074/jbc.M010346200 IS - 29 JF - J. Biol. Chem. EP - 27033 TI - RACK1 Interacts with E1A and Rescues E1A-induced Yeast Growth Inhibition and Mammalian Cell Apoptosis VL - 276 SP - 27026 KW - mammalia KW - rack1 KW - yeast AU - Sang, Nianli AU - Severino, Anna AU - Russo, Patrizia AU - Baldi, Alfonso AU - Giordano, Antonio AU - Mileo, Anna AU - Paggi, Marco AU - De Luca, Antonio PY - 2001/07/13/ UR - http://dx.doi.org/10.1074/jbc.M010346200 ER -