Functional expression cloning reveals a central role for the receptor for activated protein kinase C 1 (RACK1) in T cell apoptosis Export

@article{citeulike:3736419, abstract = {Mammalian cDNA expression cloning was used to identify novel genes that regulate apoptosis. Using a functional screen, we identified a partial cDNA for the receptor for activated protein kinase C 1 (RACK1) through selection for resistance to phytohemagglutinin and {gamma}-irradiation. Expression of this partial cDNA in T cell lines using a mammalian expression vector produced an increase in RACK1 expression and resulted in resistance to dexamethasone- and ultraviolet-induced apoptosis. Down-regulation of RACK1 using RNA interference abolished the resistance of the transfected cells to apoptosis. Overexpression of full-length RACK1 also resulted in the suppression of apoptosis mediated by several apoptotic stimuli, and this effect was quantitatively consistent with the effects of the original cDNA isolated on endogenous RACK1 levels. Together, these findings suggest that RACK1 plays an important role in the intracellular signaling pathways that lead to apoptosis in T cells. 10.1189/jlb.0205070}, author = {Mourtada-Maarabouni, Mirna and Kirkham, Lucy and Farzaneh, Farzin and Williams, Gwyn T.}, citeulike-article-id = {3736419}, citeulike-linkout-0 = {http://dx.doi.org/10.1189/jlb.0205070}, citeulike-linkout-1 = {http://www.jleukbio.org/cgi/content/abstract/78/2/503}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/15870214}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=15870214}, day = {1}, doi = {10.1189/jlb.0205070}, journal = {J Leukoc Biol}, keywords = {apoptosis, rack1}, month = {August}, number = {2}, pages = {503--514}, posted-at = {2008-12-02 16:47:00}, priority = {2}, title = {Functional expression cloning reveals a central role for the receptor for activated protein kinase C 1 (RACK1) in T cell apoptosis}, url = {http://dx.doi.org/10.1189/jlb.0205070}, volume = {78}, year = {2005} }

TY - JOUR ID - citeulike:3736419 L3 - citeulike-article-id:3736419 N2 - Mammalian cDNA expression cloning was used to identify novel genes that regulate apoptosis. Using a functional screen, we identified a partial cDNA for the receptor for activated protein kinase C 1 (RACK1) through selection for resistance to phytohemagglutinin and {gamma}-irradiation. Expression of this partial cDNA in T cell lines using a mammalian expression vector produced an increase in RACK1 expression and resulted in resistance to dexamethasone- and ultraviolet-induced apoptosis. Down-regulation of RACK1 using RNA interference abolished the resistance of the transfected cells to apoptosis. Overexpression of full-length RACK1 also resulted in the suppression of apoptosis mediated by several apoptotic stimuli, and this effect was quantitatively consistent with the effects of the original cDNA isolated on endogenous RACK1 levels. Together, these findings suggest that RACK1 plays an important role in the intracellular signaling pathways that lead to apoptosis in T cells. 10.1189/jlb.0205070 IS - 2 JF - J Leukoc Biol EP - 514 TI - Functional expression cloning reveals a central role for the receptor for activated protein kinase C 1 (RACK1) in T cell apoptosis VL - 78 SP - 503 KW - apoptosis KW - rack1 AU - Mourtada-Maarabouni, Mirna AU - Kirkham, Lucy AU - Farzaneh, Farzin AU - Williams, Gwyn PY - 2005/08/01/ UR - http://dx.doi.org/10.1189/jlb.0205070 ER -