A Ubiquitous and Conserved Signal for RNA Localization in Chordates Export

@article{citeulike:3908470, abstract = {During oogenesis in Xenopus laevis , several RNAs that localize to the vegetal cortex via one of three temporally defined pathways have been identified [ 1 , 2 , 3 and 4 ]. Although individual mRNAs utilize only one pathway, there is functional overlap [ 5 ] and apparent continuity [ 6 ] between them, suggesting that common cis -acting sequences may exist. Because previous work with the Vg1 mRNA revealed that short nontandem repeats are important for localization [ 7 and 8 ], we developed a new computer program, called REPFIND, to expedite the identification of repeated motifs in other localized RNAs. Here we show that clusters of short CAC-containing motifs characterize the localization elements (LEs) of virtually all mRNAs localized to the vegetal cortex of Xenopus oocytes. A search for this signal in GenBank [ 9 ] resulted in the identification of new localized mRNAs, demonstrating the applicability of REPFIND to predict localized RNAs. CAC-rich LEs are also found in ascidians and other vertebrates, indicating that these cis regulatory elements are conserved in chordates. Interestingly, biochemical evidence shows that distinct CAC-containing motifs have different functions in the localization process. Thus, clusters of CAC-containing motifs are a ubiquitous signal for RNA localization and can signal localization in a variety of pathways through slight variations in sequence composition.}, author = {Betley, J.}, citeulike-article-id = {3908470}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/S0960-9822(02)01220-4}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0960-9822(02)01220-4}, doi = {10.1016/S0960-9822(02)01220-4}, issn = {09609822}, journal = {Current Biology}, keywords = {method}, month = {October}, number = {20}, pages = {1756--1761}, posted-at = {2009-01-19 14:08:56}, priority = {2}, title = {A Ubiquitous and Conserved Signal for RNA Localization in Chordates}, url = {http://dx.doi.org/10.1016/S0960-9822(02)01220-4}, volume = {12}, year = {2002} }

TY - JOUR ID - citeulike:3908470 L3 - citeulike-article-id:3908470 N2 - During oogenesis in Xenopus laevis , several RNAs that localize to the vegetal cortex via one of three temporally defined pathways have been identified [ 1 , 2 , 3 and 4 ]. Although individual mRNAs utilize only one pathway, there is functional overlap [ 5 ] and apparent continuity [ 6 ] between them, suggesting that common cis -acting sequences may exist. Because previous work with the Vg1 mRNA revealed that short nontandem repeats are important for localization [ 7 and 8 ], we developed a new computer program, called REPFIND, to expedite the identification of repeated motifs in other localized RNAs. Here we show that clusters of short CAC-containing motifs characterize the localization elements (LEs) of virtually all mRNAs localized to the vegetal cortex of Xenopus oocytes. A search for this signal in GenBank [ 9 ] resulted in the identification of new localized mRNAs, demonstrating the applicability of REPFIND to predict localized RNAs. CAC-rich LEs are also found in ascidians and other vertebrates, indicating that these cis regulatory elements are conserved in chordates. Interestingly, biochemical evidence shows that distinct CAC-containing motifs have different functions in the localization process. Thus, clusters of CAC-containing motifs are a ubiquitous signal for RNA localization and can signal localization in a variety of pathways through slight variations in sequence composition. IS - 20 JF - Current Biology SN - 09609822 EP - 1761 TI - A Ubiquitous and Conserved Signal for RNA Localization in Chordates VL - 12 SP - 1756 KW - method AU - Betley, J PY - 2002/10// UR - http://dx.doi.org/10.1016/S0960-9822(02)01220-4 ER -