Enhanced Apoptosis of B and T Lymphocytes in TAFII105 Dominant-negative Transgenic Mice Is Linked to Nuclear Factor-kappa B Export

@article{citeulike:4509364, abstract = {The general transcription factor TFIID is composed of the TATA-binding protein (TBP) and 12-14 TBP-associated factors (TAFIIs). Some TAFIIs act as bridges between transcription activators and the general transcription machinery through direct interaction with activation domains. Although TAF-mediated transcription activation has been established, there is little genetic evidence connecting it to binding of an activator. TAFII105 is a substoichiometric subunit of transcription factor IID highly expressed in B lymphocytes. In this study, we examined the physiological role of TAFII105 and its mechanism of action in vivo by expressing two forms of dominant-negative mutant TAFII105 in mice. We show that TAFII105 has a pro-survival role in B and T lymphocytes, where the native protein is expressed. In addition, TAFII105 is important for T cell maturation and for production of certain antibody isotypes. These phenotypic alterations were absent in mice expressing a dominant-negative mutant that lacks one of the domains mediating p65/RelA binding in vitro. These findings provide support to the notion that interaction between the activator and TAF is important for their function in vivo. 10.1074/jbc.M200696200}, author = {Silkov, Antonina and Wolstein, Orit and Shachar, Idit and Dikstein, Rivka}, citeulike-article-id = {4509364}, citeulike-linkout-0 = {http://dx.doi.org/10.1074/jbc.M200696200}, citeulike-linkout-1 = {http://www.jbc.org/cgi/content/abstract/277/20/17821}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/11856754}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=11856754}, day = {10}, doi = {10.1074/jbc.M200696200}, journal = {J. Biol. Chem.}, keywords = {lymphocytes, mouse, nfkb, tafii105}, month = {May}, number = {20}, pages = {17821--17829}, posted-at = {2009-05-13 07:48:46}, priority = {2}, title = {Enhanced Apoptosis of B and T Lymphocytes in TAFII105 Dominant-negative Transgenic Mice Is Linked to Nuclear Factor-kappa B}, url = {http://dx.doi.org/10.1074/jbc.M200696200}, volume = {277}, year = {2002} }

TY - JOUR ID - citeulike:4509364 L3 - citeulike-article-id:4509364 N2 - The general transcription factor TFIID is composed of the TATA-binding protein (TBP) and 12-14 TBP-associated factors (TAFIIs). Some TAFIIs act as bridges between transcription activators and the general transcription machinery through direct interaction with activation domains. Although TAF-mediated transcription activation has been established, there is little genetic evidence connecting it to binding of an activator. TAFII105 is a substoichiometric subunit of transcription factor IID highly expressed in B lymphocytes. In this study, we examined the physiological role of TAFII105 and its mechanism of action in vivo by expressing two forms of dominant-negative mutant TAFII105 in mice. We show that TAFII105 has a pro-survival role in B and T lymphocytes, where the native protein is expressed. In addition, TAFII105 is important for T cell maturation and for production of certain antibody isotypes. These phenotypic alterations were absent in mice expressing a dominant-negative mutant that lacks one of the domains mediating p65/RelA binding in vitro. These findings provide support to the notion that interaction between the activator and TAF is important for their function in vivo. 10.1074/jbc.M200696200 IS - 20 JF - J. Biol. Chem. EP - 17829 TI - Enhanced Apoptosis of B and T Lymphocytes in TAFII105 Dominant-negative Transgenic Mice Is Linked to Nuclear Factor-kappa B VL - 277 SP - 17821 KW - lymphocytes KW - mouse KW - nfkb KW - tafii105 AU - Silkov, Antonina AU - Wolstein, Orit AU - Shachar, Idit AU - Dikstein, Rivka PY - 2002/05/10/ UR - http://dx.doi.org/10.1074/jbc.M200696200 ER -