"Western Blotting": Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A Export

@article{citeulike:941155, abstract = {A simple and efficient procedure was employed for the electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to sheets of pure, unmodified nitrocellulose. Immobilized proteins could then be radiographically visualized in situ by reaction with specific antibody and the subsequent binding of radioiodinated Staphylococcus protein A to the immune complexes. The detection of murine leukemia virus antigens in complex cellular lysates was used to demonstrate the efficacy of this technique.}, author = {Burnette, Neal W.}, citeulike-article-id = {941155}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/0003-2697(81)90281-5}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/0003269781902815}, citeulike-linkout-2 = {http://www.sciencedirect.com/science/article/B6W9V-4DYTNJ8-1FB/2/ab09bccc8a908a5de7a9b9779b56ded9}, doi = {10.1016/0003-2697(81)90281-5}, journal = {Analytical Biochemistry}, keywords = {method}, month = {April}, number = {2}, pages = {195--203}, posted-at = {2008-12-30 16:40:06}, priority = {2}, title = {"Western Blotting": Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A}, url = {http://dx.doi.org/10.1016/0003-2697(81)90281-5}, volume = {112}, year = {1981} }

TY - JOUR ID - citeulike:941155 L3 - citeulike-article-id:941155 N2 - A simple and efficient procedure was employed for the electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to sheets of pure, unmodified nitrocellulose. Immobilized proteins could then be radiographically visualized in situ by reaction with specific antibody and the subsequent binding of radioiodinated Staphylococcus protein A to the immune complexes. The detection of murine leukemia virus antigens in complex cellular lysates was used to demonstrate the efficacy of this technique. IS - 2 JF - Analytical Biochemistry EP - 203 TI - "Western Blotting": Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A VL - 112 SP - 195 KW - method AU - Burnette, Neal PY - 1981/04// UR - http://dx.doi.org/10.1016/0003-2697(81)90281-5 ER -