The size of the nucleus increases as yeast cells grow. Export

@article{citeulike:2326968, abstract = {It is not known how the volume of the cell nucleus is set, nor how the ratio of nuclear volume to cell volume (N/C) is determined. Here, we have measured the size of the nucleus in growing cells of the budding yeast Saccharomyces cerevisiae. Analysis of mutant yeast strains spanning a range of cell sizes revealed that the ratio of average nuclear volume to average cell volume was quite consistent, with nuclear volume being approximately 7\% that of cell volume. At the single cell level, nuclear and cell size were strongly correlated in growing wild-type cells, as determined by three different microscopic approaches. Even in G1-phase, nuclear volume grew, although it did not grow quite as fast as overall cell volume. DNA content did not appear to have any immediate, direct influence on nuclear size, in that nuclear size did not increase sharply during S-phase. The maintenance of nuclear size did not require continuous growth or ribosome biogenesis, as starvation and rapamycin treatment had little immediate impact on nuclear size. Blocking the nuclear export of new ribosomal subunits, among other proteins and RNAs, with leptomycin B also had no obvious effect on nuclear size. Nuclear expansion must now be factored into conceptual and mathematical models of budding yeast growth and division. These results raise questions as to the unknown force(s) that expand the nucleus as yeast cells grow.}, author = {Jorgensen, Paul and Edgington, Nicholas P. and Schneider, Brandt L. and Rupes, Ivan and Tyers, Mike and Futcher, Bruce}, citeulike-article-id = {2326968}, citeulike-linkout-0 = {http://dx.doi.org/10.1091/mbc.E06-10-0973}, citeulike-linkout-1 = {http://www.molbiolcell.org/cgi/content/abstract/18/9/3523}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17596521}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17596521}, day = {1}, doi = {10.1091/mbc.E06-10-0973}, issn = {1059-1524}, journal = {Molecular biology of the cell}, keywords = {growth, nucleus}, month = {September}, number = {9}, pages = {3523--3532}, posted-at = {2008-09-11 09:29:20}, priority = {2}, title = {The size of the nucleus increases as yeast cells grow.}, url = {http://dx.doi.org/10.1091/mbc.E06-10-0973}, volume = {18}, year = {2007} }

TY - JOUR ID - citeulike:2326968 L3 - citeulike-article-id:2326968 N2 - It is not known how the volume of the cell nucleus is set, nor how the ratio of nuclear volume to cell volume (N/C) is determined. Here, we have measured the size of the nucleus in growing cells of the budding yeast Saccharomyces cerevisiae. Analysis of mutant yeast strains spanning a range of cell sizes revealed that the ratio of average nuclear volume to average cell volume was quite consistent, with nuclear volume being approximately 7% that of cell volume. At the single cell level, nuclear and cell size were strongly correlated in growing wild-type cells, as determined by three different microscopic approaches. Even in G1-phase, nuclear volume grew, although it did not grow quite as fast as overall cell volume. DNA content did not appear to have any immediate, direct influence on nuclear size, in that nuclear size did not increase sharply during S-phase. The maintenance of nuclear size did not require continuous growth or ribosome biogenesis, as starvation and rapamycin treatment had little immediate impact on nuclear size. Blocking the nuclear export of new ribosomal subunits, among other proteins and RNAs, with leptomycin B also had no obvious effect on nuclear size. Nuclear expansion must now be factored into conceptual and mathematical models of budding yeast growth and division. These results raise questions as to the unknown force(s) that expand the nucleus as yeast cells grow. IS - 9 JF - Molecular biology of the cell SN - 1059-1524 EP - 3532 TI - The size of the nucleus increases as yeast cells grow. VL - 18 SP - 3523 KW - growth KW - nucleus AU - Jorgensen, Paul AU - Edgington, Nicholas AU - Schneider, Brandt AU - Rupes, Ivan AU - Tyers, Mike AU - Futcher, Bruce PY - 2007/09/01/ UR - http://dx.doi.org/10.1091/mbc.E06-10-0973 ER -