Cdc28/Cdk1 regulates spindle pole body duplication through phosphorylation of Spc42 and Mps1. Export

@article{citeulike:3860138, abstract = {Duplication of the Saccharomyces cerevisiae spindle pole body (SPB) once per cell cycle is essential for bipolar spindle formation and accurate chromosome segregation during mitosis. We have investigated the role that the major yeast cyclin-dependent kinase Cdc28/Cdk1 plays in assembly of a core SPB component, Spc42, to better understand how SPB duplication is coordinated with cell cycle progression. Cdc28 is required for SPB duplication and Spc42 assembly, and we found that Cdc28 directly phosphorylates Spc42 to promote its assembly into the SPB. The Mps1 kinase, previously shown to regulate Spc42 phosphorylation and assembly, is also a Cdc28 substrate, and Cdc28 phosphorylation of Mps1 is needed to maintain wild-type levels of Mps1 in cells. Analysis of nonphosphorylatable mutants in SPC42 and MPS1 indicates that direct Spc42 phosphorylation and indirect regulation of Spc42 through Mps1 are two overlapping pathways by which Cdc28 regulates Spc42 assembly and SPB duplication during the cell cycle.}, author = {Jaspersen, S. L. and Huneycutt, B. J. and Giddings, T. H. and Resing, K. A. and Ahn, N. G. and Winey, M.}, citeulike-article-id = {3860138}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.devcel.2004.07.006}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S1534580704002424}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/15296722}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=15296722}, doi = {10.1016/j.devcel.2004.07.006}, issn = {1534-5807}, journal = {Developmental cell}, keywords = {mps1, spc42, spindle-pole-body}, month = {August}, number = {2}, pages = {263--274}, posted-at = {2009-06-03 15:20:32}, priority = {2}, title = {Cdc28/Cdk1 regulates spindle pole body duplication through phosphorylation of Spc42 and Mps1.}, url = {http://dx.doi.org/10.1016/j.devcel.2004.07.006}, volume = {7}, year = {2004} }

TY - JOUR ID - citeulike:3860138 L3 - citeulike-article-id:3860138 N2 - Duplication of the Saccharomyces cerevisiae spindle pole body (SPB) once per cell cycle is essential for bipolar spindle formation and accurate chromosome segregation during mitosis. We have investigated the role that the major yeast cyclin-dependent kinase Cdc28/Cdk1 plays in assembly of a core SPB component, Spc42, to better understand how SPB duplication is coordinated with cell cycle progression. Cdc28 is required for SPB duplication and Spc42 assembly, and we found that Cdc28 directly phosphorylates Spc42 to promote its assembly into the SPB. The Mps1 kinase, previously shown to regulate Spc42 phosphorylation and assembly, is also a Cdc28 substrate, and Cdc28 phosphorylation of Mps1 is needed to maintain wild-type levels of Mps1 in cells. Analysis of nonphosphorylatable mutants in SPC42 and MPS1 indicates that direct Spc42 phosphorylation and indirect regulation of Spc42 through Mps1 are two overlapping pathways by which Cdc28 regulates Spc42 assembly and SPB duplication during the cell cycle. IS - 2 JF - Developmental cell SN - 1534-5807 EP - 274 TI - Cdc28/Cdk1 regulates spindle pole body duplication through phosphorylation of Spc42 and Mps1. VL - 7 SP - 263 KW - mps1 KW - spc42 KW - spindle-pole-body AU - Jaspersen, SL AU - Huneycutt, BJ AU - Giddings, TH AU - Resing, KA AU - Ahn, NG AU - Winey, M PY - 2004/08// UR - http://dx.doi.org/10.1016/j.devcel.2004.07.006 ER -