Characterization of the cyr1-2 UGA mutation in Saccharomyces cerevisiae. Export

@article{citeulike:3885668, abstract = {cyr1-2 is a temperature-sensitive mutation of the yeast adenylate cyclase structural gene, CYR1. The cyr1-2 mutation has been suggested to be a UGA mutation since a UGA suppressor SUP201 has been isolated as a suppressor of the cyr1-2 mutation. Construction of chimeric genes restricted the region containing the cyr1-2 mutation, and the cyr1-2 UGA mutation was identified at codon 1282, which lies upstream of the region coding for the catalytic domain of adenylate cyclase. Alterations in the region upstream of the cyr1-2 mutation site result in null mutations. The complete open reading frame of the cyr1-2 gene expressed under the control of the GAL1 promoter complemented cyr1-d1 in a galactose-dependent manner. These results suggest that at the permissive temperature weak readthrough occurs at the cyr1-2 mutation site to produce low levels of active adenylate cyclase. An endogenous suppressor in yeast cells is assumed to be responsible for this readthrough.}, author = {Morishita, T. and Matsuura, A. and Uno, I.}, citeulike-article-id = {3885668}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/8483461}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=8483461}, issn = {0026-8925}, journal = {Molecular \& general genetics : MGG}, keywords = {cyr1-2, cyr1-2\_uga}, month = {March}, number = {3}, pages = {463--466}, posted-at = {2009-01-14 15:43:46}, priority = {2}, title = {Characterization of the cyr1-2 UGA mutation in Saccharomyces cerevisiae.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/8483461}, volume = {237}, year = {1993} }

TY - JOUR ID - citeulike:3885668 L3 - citeulike-article-id:3885668 N2 - cyr1-2 is a temperature-sensitive mutation of the yeast adenylate cyclase structural gene, CYR1. The cyr1-2 mutation has been suggested to be a UGA mutation since a UGA suppressor SUP201 has been isolated as a suppressor of the cyr1-2 mutation. Construction of chimeric genes restricted the region containing the cyr1-2 mutation, and the cyr1-2 UGA mutation was identified at codon 1282, which lies upstream of the region coding for the catalytic domain of adenylate cyclase. Alterations in the region upstream of the cyr1-2 mutation site result in null mutations. The complete open reading frame of the cyr1-2 gene expressed under the control of the GAL1 promoter complemented cyr1-d1 in a galactose-dependent manner. These results suggest that at the permissive temperature weak readthrough occurs at the cyr1-2 mutation site to produce low levels of active adenylate cyclase. An endogenous suppressor in yeast cells is assumed to be responsible for this readthrough. IS - 3 JF - Molecular & general genetics : MGG SN - 0026-8925 EP - 466 TI - Characterization of the cyr1-2 UGA mutation in Saccharomyces cerevisiae. VL - 237 SP - 463 KW - cyr1-2 KW - cyr1-2_uga AU - Morishita, T AU - Matsuura, A AU - Uno, I PY - 1993/03// UR - http://view.ncbi.nlm.nih.gov/pubmed/8483461 ER -