Mck1, a member of the glycogen synthase kinase 3 family of protein kinases, is a negative regulator of pyruvate kinase in the yeast Saccharomyces cerevisiae. Export

@article{citeulike:3958991, abstract = {An interaction between the Saccharomyces cerevisiae protein kinase Mck1 and pyruvate kinase (Pyk1) was detected by using the two-hybrid method. Purified Mck1 was able to phosphorylate purified Pyk1 on Ser in vitro. Pyruvate kinase activity was elevated in mck1 delta cells. Several of the phenotypes of mck1 delta mutants are similar to those observed in cells overexpressing PYK1. Co-overexpression of MCK1 suppressed all of the phenotypes associated with PYK1 overexpression. These results indicate that Mck1 negatively regulates pyruvate kinase activity, possibly by direct phosphorylation.}, author = {Brazill, D. T. and Thorner, J. and Martin, G. S.}, citeulike-article-id = {3958991}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/9209064}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=9209064}, issn = {0021-9193}, journal = {Journal of bacteriology}, keywords = {mck1, pyk1}, month = {July}, number = {13}, pages = {4415--4418}, posted-at = {2009-01-27 13:22:47}, priority = {2}, title = {Mck1, a member of the glycogen synthase kinase 3 family of protein kinases, is a negative regulator of pyruvate kinase in the yeast Saccharomyces cerevisiae.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9209064}, volume = {179}, year = {1997} }

TY - JOUR ID - citeulike:3958991 L3 - citeulike-article-id:3958991 N2 - An interaction between the Saccharomyces cerevisiae protein kinase Mck1 and pyruvate kinase (Pyk1) was detected by using the two-hybrid method. Purified Mck1 was able to phosphorylate purified Pyk1 on Ser in vitro. Pyruvate kinase activity was elevated in mck1 delta cells. Several of the phenotypes of mck1 delta mutants are similar to those observed in cells overexpressing PYK1. Co-overexpression of MCK1 suppressed all of the phenotypes associated with PYK1 overexpression. These results indicate that Mck1 negatively regulates pyruvate kinase activity, possibly by direct phosphorylation. IS - 13 JF - Journal of bacteriology SN - 0021-9193 EP - 4418 TI - Mck1, a member of the glycogen synthase kinase 3 family of protein kinases, is a negative regulator of pyruvate kinase in the yeast Saccharomyces cerevisiae. VL - 179 SP - 4415 KW - mck1 KW - pyk1 AU - Brazill, DT AU - Thorner, J AU - Martin, GS PY - 1997/07// UR - http://view.ncbi.nlm.nih.gov/pubmed/9209064 ER -