The yeast MCK1 gene encodes a protein kinase homolog that activates early meiotic gene expression. Export

@article{citeulike:3958999, abstract = {We have identified a yeast gene, MCK1, that encodes a positive regulator of meiosis and spore formation. Sequence analysis revealed that MCK1 encodes a protein kinase homolog identical to YPK1, a phosphotyrosyl protein with demonstrated protein kinase activity. Increased MCK1 gene dosage accelerates the sporulation program; mck1 mutations cause delayed and decreased levels of sporulation. MCK1 is required during sporulation for maximal transcript accumulation from IME1, which encodes a meiotic activator. MCK1 is required in vegetative cells for basal IME1 expression, as evidenced by functional assays of an ime1-HIS3 fusion gene. MCK1 is also required for efficient ascus maturation. Although expression of IME1 from the GAL1 promoter restored high-level sporulation to mck1 mutants, it did not correct the ascus-maturation defect. This observation indicates that MCK1 is required, independently, for both the activation of IME1 and subsequent ascus maturation. Expression of an mck1-lacZ fusion gene was not regulated by the signals that govern meiosis. This observation is consistent with evidence that MCK1 plays a role in governing centromere function during vegetative growth as well as sporulation.}, author = {Neigeborn, L. and Mitchell, A. P.}, citeulike-article-id = {3958999}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/2010083}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=2010083}, issn = {0890-9369}, journal = {Genes \& development}, keywords = {ime1, mck1, ypk1}, month = {April}, number = {4}, pages = {533--548}, posted-at = {2009-01-27 13:34:18}, priority = {2}, title = {The yeast MCK1 gene encodes a protein kinase homolog that activates early meiotic gene expression.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/2010083}, volume = {5}, year = {1991} }

TY - JOUR ID - citeulike:3958999 L3 - citeulike-article-id:3958999 N2 - We have identified a yeast gene, MCK1, that encodes a positive regulator of meiosis and spore formation. Sequence analysis revealed that MCK1 encodes a protein kinase homolog identical to YPK1, a phosphotyrosyl protein with demonstrated protein kinase activity. Increased MCK1 gene dosage accelerates the sporulation program; mck1 mutations cause delayed and decreased levels of sporulation. MCK1 is required during sporulation for maximal transcript accumulation from IME1, which encodes a meiotic activator. MCK1 is required in vegetative cells for basal IME1 expression, as evidenced by functional assays of an ime1-HIS3 fusion gene. MCK1 is also required for efficient ascus maturation. Although expression of IME1 from the GAL1 promoter restored high-level sporulation to mck1 mutants, it did not correct the ascus-maturation defect. This observation indicates that MCK1 is required, independently, for both the activation of IME1 and subsequent ascus maturation. Expression of an mck1-lacZ fusion gene was not regulated by the signals that govern meiosis. This observation is consistent with evidence that MCK1 plays a role in governing centromere function during vegetative growth as well as sporulation. IS - 4 JF - Genes & development SN - 0890-9369 EP - 548 TI - The yeast MCK1 gene encodes a protein kinase homolog that activates early meiotic gene expression. VL - 5 SP - 533 KW - ime1 KW - mck1 KW - ypk1 AU - Neigeborn, L AU - Mitchell, AP PY - 1991/04// UR - http://view.ncbi.nlm.nih.gov/pubmed/2010083 ER -