Ady4p and Spo74p are components of the meiotic spindle pole body that promote growth of the prospore membrane in Saccharomyces cerevisiae. Export

@article{citeulike:3973109, abstract = {Spore formation in Saccharomyces cerevisiae occurs via the de novo synthesis of the prospore membrane during the second meiotic division. Prospore membrane formation is triggered by assembly of a membrane-organizing center, the meiotic outer plaque (MOP), on the cytoplasmic face of the spindle pole body (SPB) during meiosis. We report here the identification of two new components of the MOP, Ady4p and Spo74p. Ady4p and Spo74p interact with known proteins of the MOP and are localized to the outer plaque of the SPB during meiosis II. MOP assembly and prospore membrane formation are abolished in spo74Delta/spo74Delta cells and occur aberrantly in ady4Delta/ady4Delta cells. Spo74p and the MOP component Mpc70p are mutually dependent for recruitment to SPBs during meiosis. In contrast, both Ady4p and Spo74p are present at SPBs, albeit at reduced levels, in cells that lack the MOP component Mpc54p. Our findings suggest a model for the assembled MOP in which Mpc54p, Mpc70p, and Spo74p make up a core structural unit of the scaffold that initiates synthesis of the prospore membrane, and Ady4p is an auxiliary component that stabilizes the plaque.}, author = {Nickas, M. E. and Schwartz, C. and Neiman, A. M.}, citeulike-article-id = {3973109}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/12796288}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=12796288}, issn = {1535-9778}, journal = {Eukaryotic cell}, keywords = {ady4, meiosis, spindle-pole-body, spo74, sporulation}, month = {June}, number = {3}, pages = {431--445}, posted-at = {2009-01-28 11:58:38}, priority = {2}, title = {Ady4p and Spo74p are components of the meiotic spindle pole body that promote growth of the prospore membrane in Saccharomyces cerevisiae.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/12796288}, volume = {2}, year = {2003} }

TY - JOUR ID - citeulike:3973109 L3 - citeulike-article-id:3973109 N2 - Spore formation in Saccharomyces cerevisiae occurs via the de novo synthesis of the prospore membrane during the second meiotic division. Prospore membrane formation is triggered by assembly of a membrane-organizing center, the meiotic outer plaque (MOP), on the cytoplasmic face of the spindle pole body (SPB) during meiosis. We report here the identification of two new components of the MOP, Ady4p and Spo74p. Ady4p and Spo74p interact with known proteins of the MOP and are localized to the outer plaque of the SPB during meiosis II. MOP assembly and prospore membrane formation are abolished in spo74Delta/spo74Delta cells and occur aberrantly in ady4Delta/ady4Delta cells. Spo74p and the MOP component Mpc70p are mutually dependent for recruitment to SPBs during meiosis. In contrast, both Ady4p and Spo74p are present at SPBs, albeit at reduced levels, in cells that lack the MOP component Mpc54p. Our findings suggest a model for the assembled MOP in which Mpc54p, Mpc70p, and Spo74p make up a core structural unit of the scaffold that initiates synthesis of the prospore membrane, and Ady4p is an auxiliary component that stabilizes the plaque. IS - 3 JF - Eukaryotic cell SN - 1535-9778 EP - 445 TI - Ady4p and Spo74p are components of the meiotic spindle pole body that promote growth of the prospore membrane in Saccharomyces cerevisiae. VL - 2 SP - 431 KW - ady4 KW - meiosis KW - spindle-pole-body KW - spo74 KW - sporulation AU - Nickas, ME AU - Schwartz, C AU - Neiman, AM PY - 2003/06// UR - http://view.ncbi.nlm.nih.gov/pubmed/12796288 ER -