New modules for the repeated internal and N-terminal epitope tagging of genes in <I>Saccharomyces cerevisiae</I> Export

@article{citeulike:4208472, abstract = {Epitope tagging is a powerful method for the rapid analysis of protein function. In Saccharomyces cerevisiae epitope tags are introduced easily into chromosomal loci by homologous recombination using a simple PCR-based strategy. Although quite a number of tools exist for C-terminal tagging as well as N-terminal tagging of proteins expressed by heterologous promoters, there are only very limited possibilities to tag proteins at the N-terminus and retain the endogenous expression level. Furthermore, no PCR-templates for internal tagging have been reported. Here we describe new modules that are suitable for both the repeated N-terminal and internal tagging of proteins, leaving their endogenous promoters intact. The tags include 6xHA, 9xMyc, yEGFP, TEV-GST-6xHIS, ProtA, TEV-ProtA and TEV-ProtA-7xHIS in conjunction with different heterologous selection markers. Copyright {\copyright} 2004 John Wiley \& Sons, Ltd.}, address = {Max-Delbr\"{u}ck Centrum f\"{u}r Molekulare Medizin, Robert-R\"{o}ssle Str. 10, D-13092, Berlin, Germany; Friedrich Miescher Laboratorium, Max Planck Gesellschaft, Spemannstrasse 39, D-72076 T\"{u}bingen, Germany}, author = {Gauss, Robert and Trautwein, Mark and Sommer, Thomas and Spang, Anne}, citeulike-article-id = {4208472}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/yea.1187}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/109800749/ABSTRACT}, doi = {10.1002/yea.1187}, journal = {Yeast}, keywords = {cre-lox, loxp, method, n-terminal\_epitope\_tagging}, number = {1}, pages = {1--12}, posted-at = {2009-03-23 08:48:47}, priority = {2}, title = {New modules for the repeated internal and N-terminal epitope tagging of genes in <I>Saccharomyces cerevisiae</I>}, url = {http://dx.doi.org/10.1002/yea.1187}, volume = {22}, year = {2005} }

TY - JOUR ID - citeulike:4208472 L3 - citeulike-article-id:4208472 N2 - Epitope tagging is a powerful method for the rapid analysis of protein function. In Saccharomyces cerevisiae epitope tags are introduced easily into chromosomal loci by homologous recombination using a simple PCR-based strategy. Although quite a number of tools exist for C-terminal tagging as well as N-terminal tagging of proteins expressed by heterologous promoters, there are only very limited possibilities to tag proteins at the N-terminus and retain the endogenous expression level. Furthermore, no PCR-templates for internal tagging have been reported. Here we describe new modules that are suitable for both the repeated N-terminal and internal tagging of proteins, leaving their endogenous promoters intact. The tags include 6xHA, 9xMyc, yEGFP, TEV-GST-6xHIS, ProtA, TEV-ProtA and TEV-ProtA-7xHIS in conjunction with different heterologous selection markers. Copyright © 2004 John Wiley & Sons, Ltd. IS - 1 JF - Yeast AD - Max-Delbrück Centrum für Molekulare Medizin, Robert-Rössle Str. 10, D-13092, Berlin, Germany; Friedrich Miescher Laboratorium, Max Planck Gesellschaft, Spemannstrasse 39, D-72076 Tübingen, Germany EP - 12 TI - New modules for the repeated internal and N-terminal epitope tagging of genes in <I>Saccharomyces cerevisiae</I> VL - 22 SP - 1 KW - cre-lox KW - loxp KW - method KW - n-terminal_epitope_tagging AU - Gauss, Robert AU - Trautwein, Mark AU - Sommer, Thomas AU - Spang, Anne PY - 2005/// UR - http://dx.doi.org/10.1002/yea.1187 ER -