Analysis of lysine-dependent yeast sporulation: a decrease in cyclic AMP is not required for initiation of meiosis and sporulation in Saccharomyces cerevisiae Export

@article{citeulike:4920989, abstract = {Summary: Cells of the yeast Saccharomyces cerevisiae sporulated in nutrient-rich medium containing L-lysine. Sporulation was specific to the presence of L-lysine and was initiated when the cellular content of this basic amino acid reached approximately 0.2-0.5 mmol (g cells)-1, at early stationary phase. The formation of asci was most efficient at pH 7.0 and 50-100 mM L-lysine; in these optimum conditions, the sporulation frequency reached about 60\% after 5 d incubation. The L-lysine-dependent sporulation system in nutrient-rich conditions was distinct from the currently used potassium-acetate-dependent system in nutrient-deficient conditions. Analysis of the L-lysine-dependent system indicated that, prior to entrance into meiosis and/or sporulation processes, the yeast cells change in shape, their pool sizes for L-cysteine and glutathione alter, and they synthesize a protein with a molecular mass of 15 kDa. A low level of cAMP was not required for the entrance into meiosis and/or sporulation. 10.1099/13500872-141-10-2463}, author = {Suizu, Tetsuyoshi and Tsutsumi, Hiroko and Kawado, Akitsugu and Inose, Tomoko and Suginami, Koji and Murata, Kousaku}, citeulike-article-id = {4920989}, citeulike-linkout-0 = {http://dx.doi.org/10.1099/13500872-141-10-2463}, citeulike-linkout-1 = {http://mic.sgmjournals.org/cgi/content/abstract/141/10/2463?view=long&pmid=7582006}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/7582006}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=7582006}, day = {1}, doi = {10.1099/13500872-141-10-2463}, journal = {Microbiology}, keywords = {camp, lysine-dependent\_yeast\_sporulation, sporulation}, month = {October}, number = {10}, pages = {2463--2469}, posted-at = {2009-06-22 08:06:11}, priority = {2}, title = {Analysis of lysine-dependent yeast sporulation: a decrease in cyclic AMP is not required for initiation of meiosis and sporulation in Saccharomyces cerevisiae}, url = {http://dx.doi.org/10.1099/13500872-141-10-2463}, volume = {141}, year = {1995} }

TY - JOUR ID - citeulike:4920989 L3 - citeulike-article-id:4920989 N2 - Summary: Cells of the yeast Saccharomyces cerevisiae sporulated in nutrient-rich medium containing L-lysine. Sporulation was specific to the presence of L-lysine and was initiated when the cellular content of this basic amino acid reached approximately 0.2-0.5 mmol (g cells)-1, at early stationary phase. The formation of asci was most efficient at pH 7.0 and 50-100 mM L-lysine; in these optimum conditions, the sporulation frequency reached about 60% after 5 d incubation. The L-lysine-dependent sporulation system in nutrient-rich conditions was distinct from the currently used potassium-acetate-dependent system in nutrient-deficient conditions. Analysis of the L-lysine-dependent system indicated that, prior to entrance into meiosis and/or sporulation processes, the yeast cells change in shape, their pool sizes for L-cysteine and glutathione alter, and they synthesize a protein with a molecular mass of 15 kDa. A low level of cAMP was not required for the entrance into meiosis and/or sporulation. 10.1099/13500872-141-10-2463 IS - 10 JF - Microbiology EP - 2469 TI - Analysis of lysine-dependent yeast sporulation: a decrease in cyclic AMP is not required for initiation of meiosis and sporulation in Saccharomyces cerevisiae VL - 141 SP - 2463 KW - camp KW - lysine-dependent_yeast_sporulation KW - sporulation AU - Suizu, Tetsuyoshi AU - Tsutsumi, Hiroko AU - Kawado, Akitsugu AU - Inose, Tomoko AU - Suginami, Koji AU - Murata, Kousaku PY - 1995/10/01/ UR - http://dx.doi.org/10.1099/13500872-141-10-2463 ER -