A continuous electrical cell lysis device using a low dc voltage for a cell transport and rupture Export

@article{LeeCho07, abstract = {We present a continuous and low voltage cell lysis device in which a width and length of a channel change to generate focused the high electric field strength for cell lysis and the low electric field strength for a transport of samples. The previous cell lysis devices acquire the high electric field strength for a cell lysis by applying an ac voltage to a micro-gap between electrodes and require additional pumps or valves for a sample transport. However, when we change the width and length of the channel between a pair of external electrodes attached to a dc voltage, we generate both the high electric field strength for a cell lysis and the low electric field strength for an electroosmotic flow. The present device therefore performs continuous cell lysis and a sample transport without needing either an additional flow source or an additional process fabricating the electrodes for the micro-gap. The experimental study features an orifice whose width and length is 20 times narrower and 175 times shorter than the width and length of a microchannel. With an operational voltage of 50 V, the present device generates high electric field strength of 1.2 kV/cm at the orifice to disrupt cells with 100\% lysis rate of red blood cells and low electric field strength of 60 V/cm at the microchannel to generate an electroosmotic flow of 30 ± 9 ?m/s. In conclusion, the present device is capable of continuous self-pumping cell lysis at a low voltage; thus, it is suitable for a sample pretreatment component of a micro total analysis system or lab-on-a-chip. {\copyright} 2006 Elsevier B.V. All rights reserved.}, address = {Digital Nanolocomotion Center, Department of BioSystems, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon, 305-701, South Korea}, author = {Lee, D. W. and Cho, Y. H.}, citeulike-article-id = {1572701}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.snb.2006.11.054}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0925400506008033}, citeulike-linkout-2 = {http://www.scopus.com/record/display.url?view=extended&origin=resultslist&eid=2-s2.0-34248579697}, doi = {10.1016/j.snb.2006.11.054}, journal = {Sens Actuators, B Chem}, keywords = {lysis, microfluidics}, number = {1}, pages = {84--89}, posted-at = {2007-08-17 17:35:47}, priority = {2}, title = {A continuous electrical cell lysis device using a low dc voltage for a cell transport and rupture}, url = {http://dx.doi.org/10.1016/j.snb.2006.11.054}, volume = {124}, year = {2007} }

TY - JOUR ID - LeeCho07 L3 - citeulike-article-id:1572701 N2 - We present a continuous and low voltage cell lysis device in which a width and length of a channel change to generate focused the high electric field strength for cell lysis and the low electric field strength for a transport of samples. The previous cell lysis devices acquire the high electric field strength for a cell lysis by applying an ac voltage to a micro-gap between electrodes and require additional pumps or valves for a sample transport. However, when we change the width and length of the channel between a pair of external electrodes attached to a dc voltage, we generate both the high electric field strength for a cell lysis and the low electric field strength for an electroosmotic flow. The present device therefore performs continuous cell lysis and a sample transport without needing either an additional flow source or an additional process fabricating the electrodes for the micro-gap. The experimental study features an orifice whose width and length is 20 times narrower and 175 times shorter than the width and length of a microchannel. With an operational voltage of 50 V, the present device generates high electric field strength of 1.2 kV/cm at the orifice to disrupt cells with 100% lysis rate of red blood cells and low electric field strength of 60 V/cm at the microchannel to generate an electroosmotic flow of 30 ± 9 ?m/s. In conclusion, the present device is capable of continuous self-pumping cell lysis at a low voltage; thus, it is suitable for a sample pretreatment component of a micro total analysis system or lab-on-a-chip. © 2006 Elsevier B.V. All rights reserved. IS - 1 JF - Sens Actuators, B Chem AD - Digital Nanolocomotion Center, Department of BioSystems, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon, 305-701, South Korea EP - 89 TI - A continuous electrical cell lysis device using a low dc voltage for a cell transport and rupture VL - 124 SP - 84 KW - lysis KW - microfluidics AU - Lee, DW AU - Cho, YH PY - 2007/// UR - http://dx.doi.org/10.1016/j.snb.2006.11.054 ER -