Limitations of ATP estimates of microbial biomass. Export

@article{Fairbanks84, abstract = {Serious limitations were found in the use of ATP measurements as indicators of Pseudomonas paucimobilis biomass and activity in sterilized soil. These limitations were related to effects of moisture stress, nutrient variation and protozoan predation on soil ATP levels and ATP per bacterium. Rewetting dry soil resulted in an increase (2.2-fold) in ATP per bacterium, as did additions of glucose (5.9-fold) and (NH4)2SO4(2.8-fold). ATP per bacterium also differed between grazed and ungrazed bacteria. Carbon dioxide evolution and ATP concentrations varied independently with time. Thus, except under low nutrient conditions, when neither bacterial numbers nor ATP measurements changed, ATP concentrations did not indicate bacterial poulation dynamics or respiration. Carbon and N effects on ATP concentrations were reduced by adding an excess of glucose a few hours before ATP measurements.-Authors}, address = {Dept Crop \& Soil Sciences, Box 3Q, New Mexico State Univ, Las Cruces, NM 88003, USA.}, author = {Fairbanks, B. C. and Woods, L. E. and Bryant, R. J. and Elliott, E. T. and Cole, C. V. and Coleman, D. C.}, citeulike-article-id = {1574079}, citeulike-linkout-0 = {http://www.scopus.com/record/display.url?view=extended&origin=resultslist&eid=2-s2.0-0021569030}, journal = {Soil Biology and Biochemistry}, keywords = {atp, bacteria}, number = {6}, pages = {549--558}, posted-at = {2007-08-18 16:35:47}, priority = {2}, title = {Limitations of {ATP} estimates of microbial biomass.}, url = {http://www.scopus.com/record/display.url?view=extended&origin=resultslist&eid=2-s2.0-0021569030}, volume = {16}, year = {1984} }

TY - JOUR ID - Fairbanks84 L3 - citeulike-article-id:1574079 N2 - Serious limitations were found in the use of ATP measurements as indicators of Pseudomonas paucimobilis biomass and activity in sterilized soil. These limitations were related to effects of moisture stress, nutrient variation and protozoan predation on soil ATP levels and ATP per bacterium. Rewetting dry soil resulted in an increase (2.2-fold) in ATP per bacterium, as did additions of glucose (5.9-fold) and (NH4)2SO4(2.8-fold). ATP per bacterium also differed between grazed and ungrazed bacteria. Carbon dioxide evolution and ATP concentrations varied independently with time. Thus, except under low nutrient conditions, when neither bacterial numbers nor ATP measurements changed, ATP concentrations did not indicate bacterial poulation dynamics or respiration. Carbon and N effects on ATP concentrations were reduced by adding an excess of glucose a few hours before ATP measurements.-Authors IS - 6 JF - Soil Biology and Biochemistry AD - Dept Crop & Soil Sciences, Box 3Q, New Mexico State Univ, Las Cruces, NM 88003, USA. EP - 558 TI - Limitations of {ATP} estimates of microbial biomass. VL - 16 SP - 549 KW - atp KW - bacteria AU - Fairbanks, BC AU - Woods, LE AU - Bryant, RJ AU - Elliott, ET AU - Cole, CV AU - Coleman, DC PY - 1984/// UR - http://www.scopus.com/record/display.url?view=extended&origin=resultslist&eid=2-s2.0-0021569030 ER -