EDTA-induced membrane fluidization and destabilization: Biophysical studies on artificial lipid membranes Export

@article{Prachayasittikul07, abstract = {The molecular mechanism of ethylenediaminetetraacetic acid (EDTA)-induced membrane destabilization has been studied using a combination of four biophysical techniques on artificial lipid membranes. Data from Langmuir film balance and epifluorescence microscopy revealed the fluidization and expansion effect of EDTA on phase behavior of monolayers of either 1,2-dipalmitoyl-sn- glycero-3-phosphocholine (DPPC) or mixtures of DPPC and metal-chelating lipids, such as N?, N?-Bis[carboxymethyl]-N ?-[(dioctadecylamino)succinyl]-L-lysine or 1,2-dioleoyl-sn- glycero-3-[N-(5-amino-1-carboxypentyl iminodiacetic acid) succinyl]. A plausible explanation could be drawn from the electrostatic interaction between negatively charged groups of EDTA and the positively charged choline head group of DPPC. Intercalation of EDTA into the lipid membrane induced membrane curvature as elucidated by atomic force microscopy. Growth in size and shape of the membrane protrusion was found to be time-dependent upon exposure to EDTA. Further loss of material from the lipid membrane surface was monitored in real time using a quartz crystal microbalance. This indicates membrane restabilization by exclusion of the protrusions from the surface. Loss of lipid components facilitates membrane instability, leading to membrane permeabilization and lysis. {\copyright} 2007 Institute of Biochemistry and Cell Biology, SIBS, CAS.}, address = {Institute of Biochemistry, Westfa?lische Wilhelms Universita?t, Muenster 48149, Germany}, author = {Prachayasittikul, V. and Isarankura-Na-Ayudhya, C. and Tantimongcolwat, T. and Nantasenamat, C. and Galla, H. J.}, citeulike-article-id = {2269383}, citeulike-linkout-0 = {http://dx.doi.org/10.1111/j.1745-7270.2007.00350.x}, citeulike-linkout-1 = {http://www.scopus.com/record/display.url?view=extended&origin=resultslist&eid=2-s2.0-36249021386}, doi = {10.1111/j.1745-7270.2007.00350.x}, journal = {Acta Biochimica Biophysica Sinica}, keywords = {edta, lps}, number = {11}, pages = {901--913}, posted-at = {2008-01-21 16:58:36}, priority = {2}, title = {EDTA-induced membrane fluidization and destabilization: Biophysical studies on artificial lipid membranes}, url = {http://dx.doi.org/10.1111/j.1745-7270.2007.00350.x}, volume = {39}, year = {2007} }

TY - JOUR ID - Prachayasittikul07 L3 - citeulike-article-id:2269383 N2 - The molecular mechanism of ethylenediaminetetraacetic acid (EDTA)-induced membrane destabilization has been studied using a combination of four biophysical techniques on artificial lipid membranes. Data from Langmuir film balance and epifluorescence microscopy revealed the fluidization and expansion effect of EDTA on phase behavior of monolayers of either 1,2-dipalmitoyl-sn- glycero-3-phosphocholine (DPPC) or mixtures of DPPC and metal-chelating lipids, such as N?, N?-Bis[carboxymethyl]-N ?-[(dioctadecylamino)succinyl]-L-lysine or 1,2-dioleoyl-sn- glycero-3-[N-(5-amino-1-carboxypentyl iminodiacetic acid) succinyl]. A plausible explanation could be drawn from the electrostatic interaction between negatively charged groups of EDTA and the positively charged choline head group of DPPC. Intercalation of EDTA into the lipid membrane induced membrane curvature as elucidated by atomic force microscopy. Growth in size and shape of the membrane protrusion was found to be time-dependent upon exposure to EDTA. Further loss of material from the lipid membrane surface was monitored in real time using a quartz crystal microbalance. This indicates membrane restabilization by exclusion of the protrusions from the surface. Loss of lipid components facilitates membrane instability, leading to membrane permeabilization and lysis. © 2007 Institute of Biochemistry and Cell Biology, SIBS, CAS. IS - 11 JF - Acta Biochimica Biophysica Sinica AD - Institute of Biochemistry, Westfa?lische Wilhelms Universita?t, Muenster 48149, Germany EP - 913 TI - EDTA-induced membrane fluidization and destabilization: Biophysical studies on artificial lipid membranes VL - 39 SP - 901 KW - edta KW - lps AU - Prachayasittikul, V AU - Isarankura-Na-Ayudhya, C AU - Tantimongcolwat, T AU - Nantasenamat, C AU - Galla, HJ PY - 2007/// UR - http://dx.doi.org/10.1111/j.1745-7270.2007.00350.x ER -