Versatile laser-based cell manipulator Export

@article{citeulike:3137215, abstract = {Here we describe a two-photon microscope and laser ablation setup combined with optical tweezers. We tested the setup on the fission yeast Schizosaccharomyces pombe, a commonly used model organism. We show that long-term imaging can be achieved without significant photo-bleaching or damage of the sample. The setup can precisely ablate sub-micrometer structures, such as microtubules and mitotic spindles, inside living cells, which remain viable after the manipulation. Longer exposure times lead to ablation, while shorter exposures lead to photo-bleaching of the target structure. We used optical tweezers to trap intracellular particles and to displace the cell nucleus. Two-photon fluorescence imaging of the manipulated cell can be performed simultaneously with trapping. The combination of techniques described here may help to solve a variety of problems in cell biology, such as positioning of organelles and the forces exerted by the cytoskeleton. ({\copyright} 2008 WILEY-VCH Verlag GmbH \& Co. KGaA, Weinheim)}, address = {Max-Planck-Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany}, author = {Maghelli, Nicola and Toli\'{c}-N{\o}rrelykke, Iva M.}, citeulike-article-id = {3137215}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/jbio.200810026}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/120775502/ABSTRACT}, doi = {10.1002/jbio.200810026}, journal = {Journal of Biophotonics}, keywords = {microscopy, multiphoton}, number = {4}, pages = {299--309}, posted-at = {2008-08-19 17:08:29}, priority = {0}, title = {Versatile laser-based cell manipulator}, url = {http://dx.doi.org/10.1002/jbio.200810026}, volume = {1}, year = {2008} }

TY - JOUR ID - citeulike:3137215 L3 - citeulike-article-id:3137215 N2 - Here we describe a two-photon microscope and laser ablation setup combined with optical tweezers. We tested the setup on the fission yeast Schizosaccharomyces pombe, a commonly used model organism. We show that long-term imaging can be achieved without significant photo-bleaching or damage of the sample. The setup can precisely ablate sub-micrometer structures, such as microtubules and mitotic spindles, inside living cells, which remain viable after the manipulation. Longer exposure times lead to ablation, while shorter exposures lead to photo-bleaching of the target structure. We used optical tweezers to trap intracellular particles and to displace the cell nucleus. Two-photon fluorescence imaging of the manipulated cell can be performed simultaneously with trapping. The combination of techniques described here may help to solve a variety of problems in cell biology, such as positioning of organelles and the forces exerted by the cytoskeleton. (© 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) IS - 4 JF - Journal of Biophotonics AD - Max-Planck-Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany EP - 309 TI - Versatile laser-based cell manipulator VL - 1 SP - 299 KW - microscopy KW - multiphoton AU - Maghelli, Nicola AU - Tolić-Nørrelykke, Iva PY - 2008/// UR - http://dx.doi.org/10.1002/jbio.200810026 ER -