Effects of systemic resiniferatoxin treatment on substance P mRNA in rat dorsal root ganglia and substance P receptor mRNA in the spinal dorsal horn Export

@article{citeulike:500924, abstract = {Capsaicin depletes the sensory neuropeptide substance P (SP) in the rat due to a combination of neuron loss and decreased synthesis in the surviving cells. Resiniferatoxin (RTX) mimics most, but not all, capsaicin actions. In the present study, the effects of RTX (300 [mu]g/kg, s.c.) were examined on mRNA levels for SP and its receptor in the adult rat. The percentage of dorsal root ganglia (DRG) neuronal profiles showing an in situ hybridization signal for preprotachykinin mRNAs encoding SP was not altered following RTX treatment (up to 8 weeks), though the signal became perceptibly weaker. In accord, 2 weeks after RTX administration a 60\% decrease was observed in the steady-state levels of SP-encoding mRNAs using Northern blot analysis, leaving the ratio of [beta]- and [gamma]-preprotachykinin mRNAs unchanged. No change was, however, observed in mRNA levels encoding tachykinins NK-1 receptors in the dorsal horn, the spinal targets for SP. The present findings suggest that RTX does not kill SP-positive DRG neurons, though it suppresses the synthesis of SP. Since RTX treatment does not alter NK-1 receptor expression, this reduced SP synthesis is likely to play a central role in the analgesic actions of RTX.}, author = {Szallasi, Arpad and Farkas-Szallasi, Tunde and Tucker, Joseph B. and Lundberg, Jan M. and Hokfelt, Tomas and Krause, James E.}, citeulike-article-id = {500924}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/S0006-8993(98)01168-8}, citeulike-linkout-1 = {http://www.sciencedirect.com/science/article/B6SYR-3VCMSMP-2/2/c8bae9837501289f729f785d001d9361}, comment = {カプサイシンはVR1を通さない毒性もある。RTXにはない。 ---=note-separator=--- 読む。 カプサイシンに反応するレセプターは1つじゃない？ ---=note-separator=--- Capsaicin CAP-SEN-Neu Box}, day = {9}, doi = {10.1016/S0006-8993(98)01168-8}, journal = {Brain Research}, keywords = {capsaicin, drg, rat, resiniferatoxin, sp}, month = {January}, number = {2}, pages = {177--184}, posted-at = {2006-02-11 01:44:24}, priority = {4}, title = {Effects of systemic resiniferatoxin treatment on substance P mRNA in rat dorsal root ganglia and substance P receptor mRNA in the spinal dorsal horn}, url = {http://dx.doi.org/10.1016/S0006-8993(98)01168-8}, volume = {815}, year = {1999} }

TY - JOUR ID - citeulike:500924 L3 - citeulike-article-id:500924 N2 - Capsaicin depletes the sensory neuropeptide substance P (SP) in the rat due to a combination of neuron loss and decreased synthesis in the surviving cells. Resiniferatoxin (RTX) mimics most, but not all, capsaicin actions. In the present study, the effects of RTX (300 [mu]g/kg, s.c.) were examined on mRNA levels for SP and its receptor in the adult rat. The percentage of dorsal root ganglia (DRG) neuronal profiles showing an in situ hybridization signal for preprotachykinin mRNAs encoding SP was not altered following RTX treatment (up to 8 weeks), though the signal became perceptibly weaker. In accord, 2 weeks after RTX administration a 60% decrease was observed in the steady-state levels of SP-encoding mRNAs using Northern blot analysis, leaving the ratio of [beta]- and [gamma]-preprotachykinin mRNAs unchanged. No change was, however, observed in mRNA levels encoding tachykinins NK-1 receptors in the dorsal horn, the spinal targets for SP. The present findings suggest that RTX does not kill SP-positive DRG neurons, though it suppresses the synthesis of SP. Since RTX treatment does not alter NK-1 receptor expression, this reduced SP synthesis is likely to play a central role in the analgesic actions of RTX. IS - 2 JF - Brain Research EP - 184 TI - Effects of systemic resiniferatoxin treatment on substance P mRNA in rat dorsal root ganglia and substance P receptor mRNA in the spinal dorsal horn VL - 815 SP - 177 KW - capsaicin KW - drg KW - rat KW - resiniferatoxin KW - sp N1 - カプサイシンはVR1を通さない毒性もある。RTXにはない。 N1 - 読む。 カプサイシンに反応するレセプターは1つじゃない？ N1 - Capsaicin CAP-SEN-Neu Box AU - Szallasi, Arpad AU - Farkas-Szallasi, Tunde AU - Tucker, Joseph AU - Lundberg, Jan AU - Hokfelt, Tomas AU - Krause, James PY - 1999/01/09/ UR - http://dx.doi.org/10.1016/S0006-8993(98)01168-8 ER -