The effect of neonatal capsaicin treatment on the CGRP-immunoreaction in the trigeminal subnucleus caudalis of mice. Export

@article{citeulike:5030372, abstract = {The calcitonin-gene related peptide (CGRP) is a primary afferent neurotransmitter in the trigeminal system. Although a neonatal administration of capsaicin eliminates substance P (SP)-mediated nociceptive responses to induce a permanent functional reduction in C-fibers, little information is available regarding changes in CGRP-immunoreaction in mice undergoing neonatal capsaicin treatment (CP mice). This study examined postnatal changes in the distribution of CGRP-immunoreaction in the trigeminal subnucleus caudalis and trigeminal ganglion of CP mice by immunohistochemical technique and a quantitative analysis. Immunohistochemistry for CGRP in the subnucleus caudalis (Vc) demonstrated two dense distributions of neurons in the CP mice as well as na\"{i}ve mice: in the marginal layer and the region 400-600 mum deep. The quantitative analysis revealed no significant difference in the density of CGRP immunoreaction between na\"{i}ve and CP mice 1-8 weeks of age. In the trigeminal ganglion of both groups, the size distribution of CGRPpositive neurons displayed a distribution pattern with one peak in 200-300 mum(2) at week 1 and with two peaks in 200-300 mum(2) and 600-700 mum(2) at week 8 but no significant difference in neural density existed between these regions. When double staining in the na\"{i}ve mice with CGRP or SP and VR1, a capsaicin receptor, was done, many trigeminal ganglion neurons co-expressed SP- and VR1-immunoreactions, but rarely exhibited CGRP/VR1-co-localization. Taken together with previous data, these current observations suggest that CGRP containing afferent neurons possibly performs differing roles in nociceptive afferent input transmission within the Vc from SP-containing neurons in mice.}, author = {Aita, M. and Maeda, T. and Seo, K.}, citeulike-article-id = {5030372}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/18344596}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=18344596}, issn = {1880-313x}, journal = {Biomedical research (Tokyo, Japan)}, keywords = {capsaicin, cgrp, mouse, trigeminal}, month = {February}, number = {1}, pages = {33--42}, posted-at = {2009-07-01 07:18:54}, priority = {2}, title = {The effect of neonatal capsaicin treatment on the CGRP-immunoreaction in the trigeminal subnucleus caudalis of mice.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/18344596}, volume = {29}, year = {2008} }

TY - JOUR ID - citeulike:5030372 L3 - citeulike-article-id:5030372 N2 - The calcitonin-gene related peptide (CGRP) is a primary afferent neurotransmitter in the trigeminal system. Although a neonatal administration of capsaicin eliminates substance P (SP)-mediated nociceptive responses to induce a permanent functional reduction in C-fibers, little information is available regarding changes in CGRP-immunoreaction in mice undergoing neonatal capsaicin treatment (CP mice). This study examined postnatal changes in the distribution of CGRP-immunoreaction in the trigeminal subnucleus caudalis and trigeminal ganglion of CP mice by immunohistochemical technique and a quantitative analysis. Immunohistochemistry for CGRP in the subnucleus caudalis (Vc) demonstrated two dense distributions of neurons in the CP mice as well as naïve mice: in the marginal layer and the region 400-600 mum deep. The quantitative analysis revealed no significant difference in the density of CGRP immunoreaction between naïve and CP mice 1-8 weeks of age. In the trigeminal ganglion of both groups, the size distribution of CGRPpositive neurons displayed a distribution pattern with one peak in 200-300 mum(2) at week 1 and with two peaks in 200-300 mum(2) and 600-700 mum(2) at week 8 but no significant difference in neural density existed between these regions. When double staining in the naïve mice with CGRP or SP and VR1, a capsaicin receptor, was done, many trigeminal ganglion neurons co-expressed SP- and VR1-immunoreactions, but rarely exhibited CGRP/VR1-co-localization. Taken together with previous data, these current observations suggest that CGRP containing afferent neurons possibly performs differing roles in nociceptive afferent input transmission within the Vc from SP-containing neurons in mice. IS - 1 JF - Biomedical research (Tokyo, Japan) SN - 1880-313x EP - 42 TI - The effect of neonatal capsaicin treatment on the CGRP-immunoreaction in the trigeminal subnucleus caudalis of mice. VL - 29 SP - 33 KW - capsaicin KW - cgrp KW - mouse KW - trigeminal AU - Aita, M AU - Maeda, T AU - Seo, K PY - 2008/02// UR - http://view.ncbi.nlm.nih.gov/pubmed/18344596 ER -