Inhibition of cyclin-dependent kinases improves CA1 neuronal survival and behavioral performance after global ischemia in the rat. Export

@article{citeulike:3194367, abstract = {Increasing evidence suggests that cyclin-dependent kinases participate in neuronal death induced by multiple stresses in vitro. However, their role in cell death paradigms in vivo is not well characterized. Accordingly, the authors examined whether cyclin-dependent kinase inhibition resulted in functionally relevant and sustained neuroprotection in a model of global ischemia. Intracerebroventricular administration of the cyclin-dependent kinase inhibitor flavopiridol, immediately or at 4 hours postreperfusion after a global insult, reduced injury in the CA1 of the hippocampus when examined 7 days after reperfusion. No significant protection was observed when flavopiridol was administered 8 hours after reperfusion. The tumor-suppressor retinoblastoma protein, a substrate of cyclin-dependent kinase, was phosphorylated on a cyclin-dependent kinase consensus site after the global insult; this phosphorylation was inhibited by flavopiridol administration. Importantly, flavopiridol had no effect on core body temperature, suggesting that the mechanism of neuroprotection was through cyclin-dependent kinase inhibition but not through hypothermia. Furthermore, inhibition of cyclin-dependent kinases improved spatial learning behavior as assessed by the Morris water maze 7 to 9 days after reperfusion. However, the histologic protection observed at day 7 was absent 28 days after reperfusion. These results indicate that cyclin-dependent kinase inhibition provides an extended period of morphologic and functional neuroprotection that may allow time for other neuroprotective modalities to be introduced.}, address = {Neuroscience Research Institute, University of Ottawa, Ottawa, Ontario, Canada.}, author = {Wang, F. and Corbett, D. and Osuga, H. and Osuga, S. and Ikeda, J. E. and Slack, R. S. and Hogan, M. J. and Hakim, A. M. and Park, D. S.}, citeulike-article-id = {3194367}, citeulike-linkout-0 = {http://dx.doi.org/10.1097/00004647-200202000-00005}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/11823715}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=11823715}, doi = {10.1097/00004647-200202000-00005}, issn = {0271-678X}, journal = {Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism}, keywords = {apoptosis, cell, death}, month = {February}, number = {2}, pages = {171--182}, posted-at = {2008-09-04 17:02:36}, priority = {3}, title = {Inhibition of cyclin-dependent kinases improves CA1 neuronal survival and behavioral performance after global ischemia in the rat.}, url = {http://dx.doi.org/10.1097/00004647-200202000-00005}, volume = {22}, year = {2002} }

TY - JOUR ID - citeulike:3194367 L3 - citeulike-article-id:3194367 N2 - Increasing evidence suggests that cyclin-dependent kinases participate in neuronal death induced by multiple stresses in vitro. However, their role in cell death paradigms in vivo is not well characterized. Accordingly, the authors examined whether cyclin-dependent kinase inhibition resulted in functionally relevant and sustained neuroprotection in a model of global ischemia. Intracerebroventricular administration of the cyclin-dependent kinase inhibitor flavopiridol, immediately or at 4 hours postreperfusion after a global insult, reduced injury in the CA1 of the hippocampus when examined 7 days after reperfusion. No significant protection was observed when flavopiridol was administered 8 hours after reperfusion. The tumor-suppressor retinoblastoma protein, a substrate of cyclin-dependent kinase, was phosphorylated on a cyclin-dependent kinase consensus site after the global insult; this phosphorylation was inhibited by flavopiridol administration. Importantly, flavopiridol had no effect on core body temperature, suggesting that the mechanism of neuroprotection was through cyclin-dependent kinase inhibition but not through hypothermia. Furthermore, inhibition of cyclin-dependent kinases improved spatial learning behavior as assessed by the Morris water maze 7 to 9 days after reperfusion. However, the histologic protection observed at day 7 was absent 28 days after reperfusion. These results indicate that cyclin-dependent kinase inhibition provides an extended period of morphologic and functional neuroprotection that may allow time for other neuroprotective modalities to be introduced. IS - 2 JF - Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism SN - 0271-678X AD - Neuroscience Research Institute, University of Ottawa, Ottawa, Ontario, Canada. EP - 182 TI - Inhibition of cyclin-dependent kinases improves CA1 neuronal survival and behavioral performance after global ischemia in the rat. VL - 22 SP - 171 KW - apoptosis KW - cell KW - death AU - Wang, F AU - Corbett, D AU - Osuga, H AU - Osuga, S AU - Ikeda, JE AU - Slack, RS AU - Hogan, MJ AU - Hakim, AM AU - Park, DS PY - 2002/02// UR - http://dx.doi.org/10.1097/00004647-200202000-00005 ER -