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Respirometric evaluation and modeling of glucose utilization by Escherichia coli under aerobic and mesophilic cultivation conditions. Export

by: G. Insel, G. Celikyilmaz, E. Ucisik-Akkaya, K. Yesiladali, Z. P. Cakar, C. Tamerler, D. Orhon
Biotechnol Bioeng, Vol. 96, No. 1. (1 January 2007), pp. 94-105.

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adaptation diversity ecology evolution functional-groups growth model respirometry variability

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en comparant le comportement de E.coli (en souche pure ?), dans des conditions limitantes (S/X faible), le protocole experimental proposé permet de mettre en évidence une stratégie d'adaptation :

  • diminution de la vitesse de croissance (decrease mu_H) et augmentation l'affinité au substrat (decrease K_S)
  • acquisition durable de la capacité de stocker du glycogène en parallèle à la croissance.

Ces résultats sont obtenus par calage d'un modèle à des courbes de respirométrie. Le modèle utilisé n'est pas précisé dans l'abstract, de même que ce qu'il se passe au niveau des processus de stockage (en terme de modélisation et d'adaptation)

Pascalichouchou (public note) - 2008-02-04 13:58:53

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The study presents a mechanistic model for the evaluation of glucose utilization by Escherichia coli under aerobic and mesophilic growth conditions. In the first step, the experimental data was derived from batch respirometric experiments conducted at 37 degrees C, using two different initial substrate to microorganism (S(0)/X(0)) ratios of 15.0 and 1.3 mgCOD/mgSS. Acetate generation, glycogen formation and oxygen uptake rate profile were monitored together with glucose uptake and biomass increase throughout the experiments. The oxygen uptake rate (OUR) exhibited a typical profile accounting for growth on glucose, acetate and glycogen. No acetate formation (overflow) was detected at low initial S(0)/X(0) ratio. In the second step, the effect of culture history developed under long-term growth limiting conditions on the kinetics of glucose utilization by the same culture was evaluated in a sequencing batch reactor (SBR). The system was operated at cyclic steady state with a constant mean cell residence time of 5 days. The kinetic response of E.coli culture was followed by similar measurements within a complete cycle. Model calibration for the SBR system showed that E. coli culture regulated its growth metabolism by decreasing the maximum growth rate (lower microH) together with an increase of substrate affinity (lower K(S)) as compared to uncontrolled growth conditions. The continuous low rate operation of SBR system induced a significant biochemical substrate storage capability as glycogen in parallel to growth, which persisted throughout the operation. The acetate overflow was observed again as an important mechanism to be accounted for in the evaluation of process kinetics.


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