Concurrent microscopic observations and activity measurements of cellulose hydrolyzing and methanogenic populations during the batch anaerobic digestion of crystalline cellulose Export

@article{citeulike:2330335, abstract = {This study compares process data with microscopic observations from an anaerobic digestion of organic particles. As the first part of the study, this article presents detailed observations of microbial biofilm architecture and structure in a 1.25-L batch digester where all particles are of an equal age. Microcrystalline cellulose was used as the sole carbon and energy source. The digestions were inoculated with either leachate from a 220-L anaerobic municipal solid waste digester or strained rumen contents from a fistulated cow. The hydrolysis rate, when normalized by the amount of cellulose remaining in the reactor, was found to reach a constant value 1 day after inoculation with rumen fluid, and 3 days after inoculating with digester leachate. A constant value of a mass specific hydrolysis rate is argued to represent full colonization of the cellulose surface and first-order kinetics only apply after this point. Additionally, the first-order hydrolysis rate constant, once surfaces were saturated with biofilm, was found to be two times higher with a rumen inoculum, compared to a digester leachate inoculum. Images generated by fluorescence in situ hybridization (FISH) probing and confocal laser scanning microscopy show that the microbial communities involved in the anaerobic biodegradation process exist entirely within the biofilm. For the reactor conditions used in these experiments, the predominant methanogens exist in ball-shaped colonies within the biofilm. {\copyright} 2005 Wiley Periodicals, Inc.}, address = {School of Engineering, The University of Queensland, St. Lucia QLD4072, Australia, telephone: +61-7-3365-6464; fax: +61-7-3365-4199; Advance Wastewater Management Centre, The University of Queensland, St. Lucia QLD4072, Australia}, author = {Song, Hyohak and Clarke, William P. and Blackall, Linda L.}, citeulike-article-id = {2330335}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/bit.20517}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15991234}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15991234}, citeulike-linkout-3 = {http://www3.interscience.wiley.com/cgi-bin/abstract/110552571/ABSTRACT}, comment = {des donn\'{e}es sur la dynamique de colonisation de micro cristaux de cellulose, avec suivi fish et confocal. propose une interpr\'{e}tation sur la base de mod\`{e}le d'hydrolyse avec une phase d'ensemencement (0er ordre) et une phase \`{a} saturation (1er ordre du nb de cristaux) ... classique pour des questions de surface.}, doi = {10.1002/bit.20517}, journal = {Biotechnology and Bioengineering}, keywords = {anaerobic, biofilm, cellulose, colonisation, hydrolysis, in-situ, kinetics, parameter, particulate}, number = {3}, pages = {369--378}, posted-at = {2008-02-04 18:55:07}, priority = {2}, title = {Concurrent microscopic observations and activity measurements of cellulose hydrolyzing and methanogenic populations during the batch anaerobic digestion of crystalline cellulose}, url = {http://dx.doi.org/10.1002/bit.20517}, volume = {91}, year = {2005} }

TY - JOUR ID - citeulike:2330335 L3 - citeulike-article-id:2330335 N2 - This study compares process data with microscopic observations from an anaerobic digestion of organic particles. As the first part of the study, this article presents detailed observations of microbial biofilm architecture and structure in a 1.25-L batch digester where all particles are of an equal age. Microcrystalline cellulose was used as the sole carbon and energy source. The digestions were inoculated with either leachate from a 220-L anaerobic municipal solid waste digester or strained rumen contents from a fistulated cow. The hydrolysis rate, when normalized by the amount of cellulose remaining in the reactor, was found to reach a constant value 1 day after inoculation with rumen fluid, and 3 days after inoculating with digester leachate. A constant value of a mass specific hydrolysis rate is argued to represent full colonization of the cellulose surface and first-order kinetics only apply after this point. Additionally, the first-order hydrolysis rate constant, once surfaces were saturated with biofilm, was found to be two times higher with a rumen inoculum, compared to a digester leachate inoculum. Images generated by fluorescence in situ hybridization (FISH) probing and confocal laser scanning microscopy show that the microbial communities involved in the anaerobic biodegradation process exist entirely within the biofilm. For the reactor conditions used in these experiments, the predominant methanogens exist in ball-shaped colonies within the biofilm. © 2005 Wiley Periodicals, Inc. IS - 3 JF - Biotechnology and Bioengineering AD - School of Engineering, The University of Queensland, St. Lucia QLD4072, Australia, telephone: +61-7-3365-6464; fax: +61-7-3365-4199; Advance Wastewater Management Centre, The University of Queensland, St. Lucia QLD4072, Australia EP - 378 TI - Concurrent microscopic observations and activity measurements of cellulose hydrolyzing and methanogenic populations during the batch anaerobic digestion of crystalline cellulose VL - 91 SP - 369 KW - anaerobic KW - biofilm KW - cellulose KW - colonisation KW - hydrolysis KW - in-situ KW - kinetics KW - parameter KW - particulate N1 - des données sur la dynamique de colonisation de micro cristaux de cellulose, avec suivi fish et confocal. propose une interprétation sur la base de modèle d'hydrolyse avec une phase d'ensemencement (0er ordre) et une phase à saturation (1er ordre du nb de cristaux) ... classique pour des questions de surface. AU - Song, Hyohak AU - Clarke, William AU - Blackall, Linda PY - 2005/// UR - http://dx.doi.org/10.1002/bit.20517 ER -