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16S rRNA Gene Sequence Analysis Shows that Particle Size Influences Bacterial Community Structure in Aquatic Sediments Export

by: Colin R. Jackson, Andrew Q. Weeks
Appl. Environ. Microbiol. (20 June 2008), AEM.00923-08.

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Bacterial communities associated with sediment particles were examined using PCR-denaturing gradient gel electrophoresis and 16S rRNA gene sequencing. Particle size influenced community structure, with attached bacterial assemblages separating into 63-125, 125-1000, and 1000-2000 microm fractions. Differences were particularly pronounced for the Verrucomicrobia-Planctomycetes which were significantly reduced on coarser particles. 10.1128/AEM.00923-08


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