Toll-like receptor signalling in macrophages links the autophagy pathway to phagocytosis. Export

@article{citeulike:2149811, abstract = {Phagocytosis and autophagy are two ancient, highly conserved processes involved, respectively, in the removal of extracellular organisms and the destruction of organisms in the cytosol1, 2, 3. Autophagy, for either metabolic regulation or defence, involves the formation of a double membrane called the autophagosome, which then fuses with lysosomes to degrade the contents4, a process that has similarities with phagosome maturation. Toll-like-receptor (TLR) engagement activates a variety of defence mechanisms within phagocytes5, including facilitation of phagosome maturation6, and also engages autophagy7. Therefore we speculated that TLR signalling might link these processes to enhance the function of conventional phagosomes. Here we show that a particle that engages TLRs on a murine macrophage while it is phagocytosed triggers the autophagosome marker LC3 to be rapidly recruited to the phagosome in a manner that depends on the autophagy pathway proteins ATG5 and ATG7; this process is preceded by recruitment of beclin 1 and phosphoinositide-3-OH kinase activity. Translocation of beclin 1 and LC3 to the phagosome was not associated with observable double-membrane structures characteristic of conventional autophagosomes, but was associated with phagosome fusion with lysosomes, leading to rapid acidification and enhanced killing of the ingested organism.}, author = {Sanjuan, Miguel A. and Dillon, Christopher P. and Tait, Stephen W. G. and Moshiach, Simon and Dorsey, Frank and Connell, Samuel and Komatsu, Masaaki and Tanaka, Keiji and Cleveland, John L. and Withoff, Sebo and Green, Douglas R.}, citeulike-article-id = {2149811}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nature06421}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nature06421}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18097414}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18097414}, day = {20}, doi = {10.1038/nature06421}, issn = {1476-4687}, journal = {Nature}, keywords = {autophagy, beclin1, phagocytosis, signaling, tlr}, month = {December}, number = {7173}, pages = {1253--1257}, posted-at = {2008-07-30 03:11:26}, priority = {0}, publisher = {Nature Publishing Group}, title = {Toll-like receptor signalling in macrophages links the autophagy pathway to phagocytosis.}, url = {http://dx.doi.org/10.1038/nature06421}, volume = {450}, year = {2007} }

TY - JOUR ID - citeulike:2149811 L3 - citeulike-article-id:2149811 N2 - Phagocytosis and autophagy are two ancient, highly conserved processes involved, respectively, in the removal of extracellular organisms and the destruction of organisms in the cytosol1, 2, 3. Autophagy, for either metabolic regulation or defence, involves the formation of a double membrane called the autophagosome, which then fuses with lysosomes to degrade the contents4, a process that has similarities with phagosome maturation. Toll-like-receptor (TLR) engagement activates a variety of defence mechanisms within phagocytes5, including facilitation of phagosome maturation6, and also engages autophagy7. Therefore we speculated that TLR signalling might link these processes to enhance the function of conventional phagosomes. Here we show that a particle that engages TLRs on a murine macrophage while it is phagocytosed triggers the autophagosome marker LC3 to be rapidly recruited to the phagosome in a manner that depends on the autophagy pathway proteins ATG5 and ATG7; this process is preceded by recruitment of beclin 1 and phosphoinositide-3-OH kinase activity. Translocation of beclin 1 and LC3 to the phagosome was not associated with observable double-membrane structures characteristic of conventional autophagosomes, but was associated with phagosome fusion with lysosomes, leading to rapid acidification and enhanced killing of the ingested organism. IS - 7173 JF - Nature SN - 1476-4687 EP - 1257 TI - Toll-like receptor signalling in macrophages links the autophagy pathway to phagocytosis. PB - Nature Publishing Group VL - 450 SP - 1253 KW - autophagy KW - beclin1 KW - phagocytosis KW - signaling KW - tlr AU - Sanjuan, Miguel AU - Dillon, Christopher AU - Tait, Stephen AU - Moshiach, Simon AU - Dorsey, Frank AU - Connell, Samuel AU - Komatsu, Masaaki AU - Tanaka, Keiji AU - Cleveland, John AU - Withoff, Sebo AU - Green, Douglas PY - 2007/12/20/ UR - http://dx.doi.org/10.1038/nature06421 ER -