Systematic Excision of Vector Sequences from the BAC-Cloned Herpesvirus Genome during Virus Reconstitution Export

@article{citeulike:3055097, abstract = {Recently the mouse cytomegalovirus (MCMV) genome was cloned as an infectious bacterial artificial chromosome (BAC) (M. Messerle, I. Crnkovi[c'], W. Hammerschmidt, H. Ziegler, and U. H. Koszinowski, Proc. Natl. Acad. Sci. USA 94:14759-14763, 1997). The virus obtained from this construct is attenuated in vivo due to deletion of viral sequences and insertion of the BAC vector. We reconstituted the full-length MCMV genome and flanked the BAC vector with identical viral sequences. This new construct represents a versatile basis for construction of MCMV mutants since virus generated from the construct loses the bacterial sequences and acquires wild-type properties.}, author = {Wagner, Markus and Koszinowski, Ulrich H. and Messerle, Martin}, citeulike-article-id = {3055097}, citeulike-linkout-0 = {http://jvi.asm.org/cgi/content/abstract/73/8/7056}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/10400809}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=10400809}, day = {1}, journal = {J. Virol.}, keywords = {bac, genome, mcmv}, month = {August}, number = {8}, pages = {7056--7060}, posted-at = {2008-07-29 03:52:50}, priority = {2}, title = {Systematic Excision of Vector Sequences from the BAC-Cloned Herpesvirus Genome during Virus Reconstitution}, url = {http://jvi.asm.org/cgi/content/abstract/73/8/7056}, volume = {73}, year = {1999} }

TY - JOUR ID - citeulike:3055097 L3 - citeulike-article-id:3055097 N2 - Recently the mouse cytomegalovirus (MCMV) genome was cloned as an infectious bacterial artificial chromosome (BAC) (M. Messerle, I. Crnkovi[c'], W. Hammerschmidt, H. Ziegler, and U. H. Koszinowski, Proc. Natl. Acad. Sci. USA 94:14759-14763, 1997). The virus obtained from this construct is attenuated in vivo due to deletion of viral sequences and insertion of the BAC vector. We reconstituted the full-length MCMV genome and flanked the BAC vector with identical viral sequences. This new construct represents a versatile basis for construction of MCMV mutants since virus generated from the construct loses the bacterial sequences and acquires wild-type properties. IS - 8 JF - J. Virol. EP - 7060 TI - Systematic Excision of Vector Sequences from the BAC-Cloned Herpesvirus Genome during Virus Reconstitution VL - 73 SP - 7056 KW - bac KW - genome KW - mcmv AU - Wagner, Markus AU - Koszinowski, Ulrich AU - Messerle, Martin PY - 1999/08/01/ UR - http://jvi.asm.org/cgi/content/abstract/73/8/7056 ER -