Cloning and mutagenesis of a herpesvirus genome as an infectious bacterial artificial chromosome Export

@article{citeulike:3055098, abstract = {A strategy for cloning and mutagenesis of an infectious herpesvirus genome is described. The mouse cytomegalovirus genome was cloned and maintained as a 230 kb bacterial artificial chromosome (BAC) in E. coli. Transfection of the BAC plasmid into eukaryotic cells led to a productive virus infection. The feasibility to introduce targeted mutations into the BAC cloned virus genome was shown by mutation of the immediate-early 1 gene and generation of a mutant virus. Thus, the complete construction of a mutant herpesvirus genome can now be carried out in a controlled manner prior to the reconstitution of infectious progeny. The described approach should be generally applicable to the mutagenesis of genomes of other large DNA viruses.}, author = {Messerle, Martin and Crnkovic, Irena and Hammerschmidt, Wolfgang and Ziegler, Heike and Koszinowski, Ulrich H.}, citeulike-article-id = {3055098}, citeulike-linkout-0 = {http://www.pnas.org/content/94/26/14759}, day = {23}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {bac, genome, mcmv}, month = {December}, number = {26}, pages = {14759--14763}, posted-at = {2008-07-29 03:55:48}, priority = {2}, title = {Cloning and mutagenesis of a herpesvirus genome as an infectious bacterial artificial chromosome}, url = {http://www.pnas.org/content/94/26/14759}, volume = {94}, year = {1997} }

TY - JOUR ID - citeulike:3055098 L3 - citeulike-article-id:3055098 N2 - A strategy for cloning and mutagenesis of an infectious herpesvirus genome is described. The mouse cytomegalovirus genome was cloned and maintained as a 230 kb bacterial artificial chromosome (BAC) in E. coli. Transfection of the BAC plasmid into eukaryotic cells led to a productive virus infection. The feasibility to introduce targeted mutations into the BAC cloned virus genome was shown by mutation of the immediate-early 1 gene and generation of a mutant virus. Thus, the complete construction of a mutant herpesvirus genome can now be carried out in a controlled manner prior to the reconstitution of infectious progeny. The described approach should be generally applicable to the mutagenesis of genomes of other large DNA viruses. IS - 26 JF - Proceedings of the National Academy of Sciences of the United States of America EP - 14763 TI - Cloning and mutagenesis of a herpesvirus genome as an infectious bacterial artificial chromosome VL - 94 SP - 14759 KW - bac KW - genome KW - mcmv AU - Messerle, Martin AU - Crnkovic, Irena AU - Hammerschmidt, Wolfgang AU - Ziegler, Heike AU - Koszinowski, Ulrich PY - 1997/12/23/ UR - http://www.pnas.org/content/94/26/14759 ER -