CpG motifs in bacterial DNA trigger direct B-cell activation Export

@article{citeulike:3055170, abstract = {Unmethylated CpG dinucleotides are more frequent in the genomes of bacteria and viruses than of vertebrates. We report here that bacterial DNA and synthetic oligodeoxynucleotides containing unmethylated CpG dinucleotides induce murine B cells to proliferate and secrete immunoglobulin in vitro and in vivo. This activation is enhanced by simultaneous signals delivered through the antigen receptor. Optimal B-cell activation requires a DNA motif in which an unmethylated CpG dinucleotide is flanked by two 5' purines and two 3' pyrimidines. Oligodeoxynucleotides containing this CpG motif induce more than 95\% of all spleen B cells to enter the cell cycle. These data suggest a possible evolutionary link between immune defence based on the recognition of microbial DNA and the phenomenon of 'CpG suppression' in vertebrates. The potent immune activation by CpG oligonucleotides has implications for the design and interpretation of studies using 'antisense' oligonucleotides and points to possible new applications as adjuvants.}, author = {Krieg, Arthur M. and Yi, Ae-Kyung and Matson, Sara and Waldschmidt, Thomas J. and Bishop, Gail A. and Teasdale, Rebecca and Koretzky, Gary A. and Klinman, Dennis M.}, citeulike-article-id = {3055170}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/374546a0}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/374546a0}, day = {6}, doi = {10.1038/374546a0}, journal = {Nature}, keywords = {antibody, b\_cells, bacteria, cpg, immunostimulatory}, month = {April}, number = {6522}, pages = {546--549}, posted-at = {2008-07-29 04:52:55}, priority = {0}, title = {CpG motifs in bacterial DNA trigger direct B-cell activation}, url = {http://dx.doi.org/10.1038/374546a0}, volume = {374}, year = {1995} }

TY - JOUR ID - citeulike:3055170 L3 - citeulike-article-id:3055170 N2 - Unmethylated CpG dinucleotides are more frequent in the genomes of bacteria and viruses than of vertebrates. We report here that bacterial DNA and synthetic oligodeoxynucleotides containing unmethylated CpG dinucleotides induce murine B cells to proliferate and secrete immunoglobulin in vitro and in vivo. This activation is enhanced by simultaneous signals delivered through the antigen receptor. Optimal B-cell activation requires a DNA motif in which an unmethylated CpG dinucleotide is flanked by two 5' purines and two 3' pyrimidines. Oligodeoxynucleotides containing this CpG motif induce more than 95% of all spleen B cells to enter the cell cycle. These data suggest a possible evolutionary link between immune defence based on the recognition of microbial DNA and the phenomenon of 'CpG suppression' in vertebrates. The potent immune activation by CpG oligonucleotides has implications for the design and interpretation of studies using 'antisense' oligonucleotides and points to possible new applications as adjuvants. IS - 6522 JF - Nature EP - 549 TI - CpG motifs in bacterial DNA trigger direct B-cell activation VL - 374 SP - 546 KW - antibody KW - b_cells KW - bacteria KW - cpg KW - immunostimulatory AU - Krieg, Arthur AU - Yi, Ae-Kyung AU - Matson, Sara AU - Waldschmidt, Thomas AU - Bishop, Gail AU - Teasdale, Rebecca AU - Koretzky, Gary AU - Klinman, Dennis PY - 1995/04/06/ UR - http://dx.doi.org/10.1038/374546a0 ER -