Vasopressin V2 receptor expression along rat, mouse, and human renal epithelia with focus on TAL. Export

@article{citeulike:2926027, abstract = {In renal epithelia, vasopressin influences salt and water transport, chiefly via vasopressin V(2) receptors (V(2)Rs) linked to adenylyl cyclase. A combination of vasopressin-induced effects along several distinct portions of the nephron and collecting duct system may help balance the net effects of antidiuresis in cortex and medulla. Previous studies of the intrarenal distribution of V(2)Rs have been inconclusive with respect to segment- and cell-type-related V(2)R expression. Our study therefore aimed to present a high-resolution analysis of V(2)R mRNA expression in rat, mouse, and human kidney epithelia, supplemented with immunohistochemical data. Cell types of the renal tubule were identified histochemically using specific markers. Pronounced V(2)R signal in thick ascending limb (TAL) was corroborated functionally; phosphorylation of Na(+)-K(+)-2Cl(-) cotransporter type 2 (NKCC2) was established in cultured TAL cells from rabbit and in rats with diabetes insipidus that were treated with the V(2)R agonist desmopressin. We found solid expression of V(2)R mRNA in medullary TAL (MTAL), macula densa, connecting tubule, and cortical and medullary collecting duct and weaker expression in cortical TAL and distal convoluted tubule in all three species. Additional V(2)R immunostaining of kidneys and rabbit TAL cells confirmed our findings. In agreement with strong V(2)R expression in MTAL, kidneys from rats with diabetes insipidus and cultured TAL cells revealed sharp, selective increases in NKCC2 phosphorylation upon desmopressin treatment. Macula densa cells constitutively showed strong NKCC2 phosphorylation. Results suggest comparably significant effects of vasopressin-induced V(2)R signaling in MTAL and in connecting tubule/collecting duct principal cells across the three species. Strong V(2)R expression in macula densa may be related to tubulovascular signal transfer.}, address = {Department of Anatomy, Charit\'{e} Universit\"{a}tsmedizin, Berlin, Germany.}, author = {Mutig, K. and Paliege, A. and Kahl, T. and J\"{o}ns, T. and M\"{u}ller-Esterl, W. and Bachmann, S.}, citeulike-article-id = {2926027}, citeulike-linkout-0 = {http://dx.doi.org/10.1152/ajprenal.00196.2007}, citeulike-linkout-1 = {http://ajprenal.physiology.org/cgi/content/abstract/293/4/F1166}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17626156}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17626156}, doi = {10.1152/ajprenal.00196.2007}, issn = {0363-6127}, journal = {American journal of physiology. Renal physiology}, keywords = {receptor, v2}, month = {October}, number = {4}, posted-at = {2009-04-15 14:39:02}, priority = {2}, title = {Vasopressin V2 receptor expression along rat, mouse, and human renal epithelia with focus on TAL.}, url = {http://dx.doi.org/10.1152/ajprenal.00196.2007}, volume = {293}, year = {2007} }

TY - JOUR ID - citeulike:2926027 L3 - citeulike-article-id:2926027 N2 - In renal epithelia, vasopressin influences salt and water transport, chiefly via vasopressin V(2) receptors (V(2)Rs) linked to adenylyl cyclase. A combination of vasopressin-induced effects along several distinct portions of the nephron and collecting duct system may help balance the net effects of antidiuresis in cortex and medulla. Previous studies of the intrarenal distribution of V(2)Rs have been inconclusive with respect to segment- and cell-type-related V(2)R expression. Our study therefore aimed to present a high-resolution analysis of V(2)R mRNA expression in rat, mouse, and human kidney epithelia, supplemented with immunohistochemical data. Cell types of the renal tubule were identified histochemically using specific markers. Pronounced V(2)R signal in thick ascending limb (TAL) was corroborated functionally; phosphorylation of Na(+)-K(+)-2Cl(-) cotransporter type 2 (NKCC2) was established in cultured TAL cells from rabbit and in rats with diabetes insipidus that were treated with the V(2)R agonist desmopressin. We found solid expression of V(2)R mRNA in medullary TAL (MTAL), macula densa, connecting tubule, and cortical and medullary collecting duct and weaker expression in cortical TAL and distal convoluted tubule in all three species. Additional V(2)R immunostaining of kidneys and rabbit TAL cells confirmed our findings. In agreement with strong V(2)R expression in MTAL, kidneys from rats with diabetes insipidus and cultured TAL cells revealed sharp, selective increases in NKCC2 phosphorylation upon desmopressin treatment. Macula densa cells constitutively showed strong NKCC2 phosphorylation. Results suggest comparably significant effects of vasopressin-induced V(2)R signaling in MTAL and in connecting tubule/collecting duct principal cells across the three species. Strong V(2)R expression in macula densa may be related to tubulovascular signal transfer. IS - 4 JF - American journal of physiology. Renal physiology SN - 0363-6127 AD - Department of Anatomy, Charité Universitätsmedizin, Berlin, Germany. TI - Vasopressin V2 receptor expression along rat, mouse, and human renal epithelia with focus on TAL. VL - 293 KW - receptor KW - v2 AU - Mutig, K AU - Paliege, A AU - Kahl, T AU - Jöns, T AU - Müller-Esterl, W AU - Bachmann, S PY - 2007/10// UR - http://dx.doi.org/10.1152/ajprenal.00196.2007 ER -