Essential genes of a minimal bacterium Export

@article{citeulike:464453, abstract = {10.1073/pnas.0510013103 has the smallest genome of any organism that can be grown in pure culture. It has a minimal metabolism and little genomic redundancy. Consequently, its genome is expected to be a close approximation to the minimal set of genes needed to sustain bacterial life. Using global transposon mutagenesis, we isolated and characterized gene disruption mutants for 100 different nonessential protein-coding genes. None of the 43 RNA-coding genes were disrupted. Herein, we identify 382 of the 482 protein-coding genes as essential, plus five sets of disrupted genes that encode proteins with potentially redundant essential functions, such as phosphate transport. Genes encoding proteins of unknown function constitute 28\% of the essential protein-coding genes set. Disruption of some genes accelerated growth.}, author = {Glass, John I. and Assad-Garcia, Nacyra and Alperovich, Nina and Yooseph, Shibu and Lewis, Matthew R. and Maruf, Mahir and Hutchison, Clyde A. and Smith, Hamilton O. and Venter, J. Craig}, citeulike-article-id = {464453}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0510013103}, citeulike-linkout-1 = {http://www.pnas.org/content/103/2/425.full.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/103/2/425.full.full.pdf}, citeulike-linkout-3 = {http://www.pnas.org/cgi/content/abstract/103/2/425}, citeulike-linkout-4 = {http://view.ncbi.nlm.nih.gov/pubmed/16407165}, citeulike-linkout-5 = {http://www.hubmed.org/display.cgi?uids=16407165}, day = {10}, doi = {10.1073/pnas.0510013103}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {algorithmic-information-theory, complexities, compressability, compressibility, extremal-biology, genes, genome-spaces, minimal-models, minimum-suffcient-models, model-organisms, prototypes, simplicity, sufficiency}, month = {January}, number = {2}, pages = {425--430}, posted-at = {2009-06-08 12:07:29}, priority = {2}, title = {Essential genes of a minimal bacterium}, url = {http://dx.doi.org/10.1073/pnas.0510013103}, volume = {103}, year = {2006} }

TY - JOUR ID - citeulike:464453 L3 - citeulike-article-id:464453 N2 - 10.1073/pnas.0510013103 has the smallest genome of any organism that can be grown in pure culture. It has a minimal metabolism and little genomic redundancy. Consequently, its genome is expected to be a close approximation to the minimal set of genes needed to sustain bacterial life. Using global transposon mutagenesis, we isolated and characterized gene disruption mutants for 100 different nonessential protein-coding genes. None of the 43 RNA-coding genes were disrupted. Herein, we identify 382 of the 482 protein-coding genes as essential, plus five sets of disrupted genes that encode proteins with potentially redundant essential functions, such as phosphate transport. Genes encoding proteins of unknown function constitute 28% of the essential protein-coding genes set. Disruption of some genes accelerated growth. IS - 2 JF - Proceedings of the National Academy of Sciences of the United States of America EP - 430 TI - Essential genes of a minimal bacterium VL - 103 SP - 425 KW - algorithmic-information-theory KW - complexities KW - compressability KW - compressibility KW - extremal-biology KW - genes KW - genome-spaces KW - minimal-models KW - minimum-suffcient-models KW - model-organisms KW - prototypes KW - simplicity KW - sufficiency AU - Glass, John AU - Assad-Garcia, Nacyra AU - Alperovich, Nina AU - Yooseph, Shibu AU - Lewis, Matthew AU - Maruf, Mahir AU - Hutchison, Clyde AU - Smith, Hamilton AU - Venter, Craig PY - 2006/01/10/ UR - http://dx.doi.org/10.1073/pnas.0510013103 ER -