Characterization of human embryonic stem cell lines by the International Stem Cell Initiative. Export

@article{citeulike:1401964, abstract = {The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue-nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected.}, address = {UK Stem Cell Bank, Division of Cell Biology and Imaging, National Institute for Biological Standards and Control, South Mimms, Herts., EN6 3QG, UK.}, author = {{} and Adewumi, Oluseun and Aflatoonian, Behrouz and Ahrlund-Richter, Lars and Amit, Michal and Andrews, Peter W W. and Beighton, Gemma and Bello, Paul A A. and Benvenisty, Nissim and Berry, Lorraine S S. and Bevan, Simon and Blum, Barak and Brooking, Justin and Chen, Kevin G G. and Choo, Andre B H B. and Churchill, Gary A A. and Corbel, Marie and Damjanov, Ivan and Draper, Jon S S. and Dvorak, Petr and Emanuelsson, Katarina and Fleck, Roland A A. and Ford, Angela and Gertow, Karin and Gertsenstein, Marina and Gokhale, Paul J J. and Hamilton, Rebecca S S. and Hampl, Ales and Healy, Lyn E E. and Hovatta, Outi and Hyllner, Johan and Imreh, Marta P P. and Itskovitz-Eldor, Joseph and Jackson, Jamie and Johnson, Jacqueline L L. and Jones, Mark and Kee, Kehkooi and King, Benjamin L L. and Knowles, Barbara B B. and Lako, Majlinda and Lebrin, Franck and Mallon, Barbara S S. and Manning, Daisy and Mayshar, Yoav and McKay, Ronald D G D. and Michalska, Anna E E. and Mikkola, Milla and Mileikovsky, Masha and Minger, Stephen L L. and Moore, Harry D D. and Mummery, Christine L L. and Nagy, Andras and Nakatsuji, Norio and O'brien, Carmel M M. and Oh, Steve K W K. and Olsson, Cia and Otonkoski, Timo and Park, Kye-Yoon Y. and Passier, Robert and Patel, Hema and Patel, Minal and Pedersen, Roger and Pera, Martin F F. and Piekarczyk, Marian S S. and Pera, Renee A Reijo A. and Reubinoff, Benjamin E E. and Robins, Allan J J. and Rossant, Janet and Rugg-Gunn, Peter and Schulz, Thomas C C. and Semb, Henrik and Sherrer, Eric S S. and Siemen, Henrike and Stacey, Glyn N N. and Stojkovic, Miodrag and Suemori, Hirofumi and Szatkiewicz, Jin and Turetsky, Tikva and Tuuri, Timo and van den Brink, Steineke and Vintersten, Kristina and Vuoristo, Sanna and Ward, Dorien and Weaver, Thomas A A. and Young, Lesley A A. and Zhang, Weidong}, citeulike-article-id = {1401964}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nbt1318}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17572666}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17572666}, doi = {10.1038/nbt1318}, issn = {1087-0156}, journal = {Nat Biotechnol}, month = {June}, posted-at = {2007-06-21 08:16:21}, priority = {2}, title = {Characterization of human embryonic stem cell lines by the International Stem Cell Initiative.}, url = {http://dx.doi.org/10.1038/nbt1318}, year = {2007} }

TY - JOUR ID - citeulike:1401964 L3 - citeulike-article-id:1401964 N2 - The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue-nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected. JF - Nat Biotechnol SN - 1087-0156 AD - UK Stem Cell Bank, Division of Cell Biology and Imaging, National Institute for Biological Standards and Control, South Mimms, Herts., EN6 3QG, UK. TI - Characterization of human embryonic stem cell lines by the International Stem Cell Initiative. AU - Adewumi, Oluseun AU - Aflatoonian, Behrouz AU - Ahrlund-Richter, Lars AU - Amit, Michal AU - Andrews, Peter W AU - Beighton, Gemma AU - Bello, Paul A AU - Benvenisty, Nissim AU - Berry, Lorraine S AU - Bevan, Simon AU - Blum, Barak AU - Brooking, Justin AU - Chen, Kevin G AU - Choo, Andre B H AU - Churchill, Gary A AU - Corbel, Marie AU - Damjanov, Ivan AU - Draper, Jon S AU - Dvorak, Petr AU - Emanuelsson, Katarina AU - Fleck, Roland A AU - Ford, Angela AU - Gertow, Karin AU - Gertsenstein, Marina AU - Gokhale, Paul J AU - Hamilton, Rebecca S AU - Hampl, Ales AU - Healy, Lyn E AU - Hovatta, Outi AU - Hyllner, Johan AU - Imreh, Marta P AU - Itskovitz-Eldor, Joseph AU - Jackson, Jamie AU - Johnson, Jacqueline L AU - Jones, Mark AU - Kee, Kehkooi AU - King, Benjamin L AU - Knowles, Barbara B AU - Lako, Majlinda AU - Lebrin, Franck AU - Mallon, Barbara S AU - Manning, Daisy AU - Mayshar, Yoav AU - McKay, Ronald D G AU - Michalska, Anna E AU - Mikkola, Milla AU - Mileikovsky, Masha AU - Minger, Stephen L AU - Moore, Harry D AU - Mummery, Christine L AU - Nagy, Andras AU - Nakatsuji, Norio AU - O'brien, Carmel M AU - Oh, Steve K W AU - Olsson, Cia AU - Otonkoski, Timo AU - Park, Kye-Yoon AU - Passier, Robert AU - Patel, Hema AU - Patel, Minal AU - Pedersen, Roger AU - Pera, Martin F AU - Piekarczyk, Marian S AU - Pera, Renee A Reijo AU - Reubinoff, Benjamin E AU - Robins, Allan J AU - Rossant, Janet AU - Rugg-Gunn, Peter AU - Schulz, Thomas C AU - Semb, Henrik AU - Sherrer, Eric S AU - Siemen, Henrike AU - Stacey, Glyn N AU - Stojkovic, Miodrag AU - Suemori, Hirofumi AU - Szatkiewicz, Jin AU - Turetsky, Tikva AU - Tuuri, Timo AU - van den Brink, Steineke AU - Vintersten, Kristina AU - Vuoristo, Sanna AU - Ward, Dorien AU - Weaver, Thomas A AU - Young, Lesley A AU - Zhang, Weidong PY - 2007/06/17/ UR - http://dx.doi.org/10.1038/nbt1318 ER -