Human CD8+ T Cell Memory Generation in Puumala Hantavirus Infection Occurs after the Acute Phase and Is Associated with Boosting of EBV-Specific CD8+ Memory T Cells. Export

@article{citeulike:1476456, abstract = {The induction and maintenance of T cell memory is incompletely understood, especially in humans. We have studied the T cell response and the generation of memory during acute infection by the Puumala virus (PUUV), a hantavirus endemic to Europe. It causes a self-limiting infection with no viral persistence, manifesting as hemorrhagic fever with renal syndrome. HLA tetramer staining of PBMC showed that the CD8(+) T cell response peaked at the onset of the clinical disease and decreased within the next 3 wk. Expression of activation markers on the tetramer-positive T cells was also highest during the acute phase, suggesting that the peak population consisted largely of effector cells. Despite the presence of tetramer-positive T cells expressing cytoplasmic IFN-gamma, PUUV-specific cells producing IFN-gamma in vitro were rare during the acute phase. Their frequency, as well as the expression of IL-7Ralpha mRNA and surface protein, increased during a follow-up period of 6 wk and probably reflected the induction of memory T cells. Simultaneously with the PUUV-specific response, we also noted in seven of nine patients an increase in EBV-specific T cells and the transient presence of EBV DNA in three patients, indicative of viral reactivation. Our results show that in a natural human infection CD8(+) memory T cells are rare during the peak response, gradually emerging during the first weeks of convalescence. They also suggest that the boosting of unrelated memory T cells may be a common occurrence in human viral infections, which may have significant implications for the homeostasis of the memory T cell compartment.}, address = {Haartman Institute.}, author = {Tuuminen, T. and Kek\"{a}l\"{a}inen, E. and M\"{a}kel\"{a}, S. and Ala-Houhala, I. and Ennis, F. A. and Hedman, K. and Mustonen, J. and Vaheri, A. and Arstila, T. P.}, citeulike-article-id = {1476456}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/17641066}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=17641066}, day = {1}, issn = {0022-1767}, journal = {J Immunol}, month = {August}, number = {3}, pages = {1988--1995}, posted-at = {2007-07-24 10:51:34}, priority = {2}, title = {Human CD8+ T Cell Memory Generation in Puumala Hantavirus Infection Occurs after the Acute Phase and Is Associated with Boosting of EBV-Specific CD8+ Memory T Cells.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/17641066}, volume = {179}, year = {2007} }

TY - JOUR ID - citeulike:1476456 L3 - citeulike-article-id:1476456 N2 - The induction and maintenance of T cell memory is incompletely understood, especially in humans. We have studied the T cell response and the generation of memory during acute infection by the Puumala virus (PUUV), a hantavirus endemic to Europe. It causes a self-limiting infection with no viral persistence, manifesting as hemorrhagic fever with renal syndrome. HLA tetramer staining of PBMC showed that the CD8(+) T cell response peaked at the onset of the clinical disease and decreased within the next 3 wk. Expression of activation markers on the tetramer-positive T cells was also highest during the acute phase, suggesting that the peak population consisted largely of effector cells. Despite the presence of tetramer-positive T cells expressing cytoplasmic IFN-gamma, PUUV-specific cells producing IFN-gamma in vitro were rare during the acute phase. Their frequency, as well as the expression of IL-7Ralpha mRNA and surface protein, increased during a follow-up period of 6 wk and probably reflected the induction of memory T cells. Simultaneously with the PUUV-specific response, we also noted in seven of nine patients an increase in EBV-specific T cells and the transient presence of EBV DNA in three patients, indicative of viral reactivation. Our results show that in a natural human infection CD8(+) memory T cells are rare during the peak response, gradually emerging during the first weeks of convalescence. They also suggest that the boosting of unrelated memory T cells may be a common occurrence in human viral infections, which may have significant implications for the homeostasis of the memory T cell compartment. IS - 3 JF - J Immunol SN - 0022-1767 AD - Haartman Institute. EP - 1995 TI - Human CD8+ T Cell Memory Generation in Puumala Hantavirus Infection Occurs after the Acute Phase and Is Associated with Boosting of EBV-Specific CD8+ Memory T Cells. VL - 179 SP - 1988 AU - Tuuminen, T AU - Kekäläinen, E AU - Mäkelä, S AU - Ala-Houhala, I AU - Ennis, FA AU - Hedman, K AU - Mustonen, J AU - Vaheri, A AU - Arstila, TP PY - 2007/08/01/ UR - http://view.ncbi.nlm.nih.gov/pubmed/17641066 ER -