Isolation of human embryonic stem cell-derived teratomas for the assessment of pluripotency. Export

@article{citeulike:3271213, abstract = {This unit describes protocols on how to assess the developmental potency of human embryonic stem cells (hESCs) by performing xenografting into immunodeficient mice to induce teratoma formation. hESCs can be injected under the testis capsule, or alternatively into the kidney or subcutaneously. Teratomas that develop from grafted hESCs are surgically removed, fixed in formaldehyde, and paraffin embedded. The tissues in the teratoma are analyzed histologically to determine whether the hESCs are pluripotent and form tissues derived from of all three embryonic germ layers (ectoderm, mesoderm, and endoderm). Teratomas can also be fixed in Bouin's or cryosectioned for analysis, and they can be analyzed by immunohistochemistry for tissue markers. Methods for these procedures are included in this unit.}, address = {Department of Laboratory Medicine, Clinical Research Center, Unit for Molecular Embryology, Karolinska Institute, Sweden.}, author = {Gertow, K. and Przyborski, S. and Loring, J. F. and Auerbach, J. M. and Epifano, O. and Otonkoski, T. and Damjanov, I. and Ahrlund-Richter, L.}, citeulike-article-id = {3271213}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/9780470151808.sc01b04s3}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18785162}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18785162}, doi = {10.1002/9780470151808.sc01b04s3}, issn = {1938-8969}, journal = {Current protocols in stem cell biology}, month = {October}, posted-at = {2008-09-16 08:07:49}, priority = {2}, title = {Isolation of human embryonic stem cell-derived teratomas for the assessment of pluripotency.}, url = {http://dx.doi.org/10.1002/9780470151808.sc01b04s3}, volume = {Chapter 1}, year = {2007} }

TY - JOUR ID - citeulike:3271213 L3 - citeulike-article-id:3271213 N2 - This unit describes protocols on how to assess the developmental potency of human embryonic stem cells (hESCs) by performing xenografting into immunodeficient mice to induce teratoma formation. hESCs can be injected under the testis capsule, or alternatively into the kidney or subcutaneously. Teratomas that develop from grafted hESCs are surgically removed, fixed in formaldehyde, and paraffin embedded. The tissues in the teratoma are analyzed histologically to determine whether the hESCs are pluripotent and form tissues derived from of all three embryonic germ layers (ectoderm, mesoderm, and endoderm). Teratomas can also be fixed in Bouin's or cryosectioned for analysis, and they can be analyzed by immunohistochemistry for tissue markers. Methods for these procedures are included in this unit. JF - Current protocols in stem cell biology SN - 1938-8969 AD - Department of Laboratory Medicine, Clinical Research Center, Unit for Molecular Embryology, Karolinska Institute, Sweden. TI - Isolation of human embryonic stem cell-derived teratomas for the assessment of pluripotency. VL - Chapter 1 AU - Gertow, K AU - Przyborski, S AU - Loring, JF AU - Auerbach, JM AU - Epifano, O AU - Otonkoski, T AU - Damjanov, I AU - Ahrlund-Richter, L PY - 2007/10// UR - http://dx.doi.org/10.1002/9780470151808.sc01b04s3 ER -