Stromal laminin chain distribution in normal, hyperplastic and malignant oral mucosa: relation to myofibroblast occurrence and vessel formation. Export

@article{citeulike:6097087, abstract = {Background: The contribution of stromal laminin chain expression to malignant potential, tumour stroma reorganization and vessel formation in oral squamous cell carcinoma (OSCC) is not fully understood. Therefore, the expression of the laminin chains alpha2, alpha3, alpha4, alpha5 and gamma2 in the stromal compartment/vascular structures in OSCC was analysed. Methods: Frozen tissue of OSCC (9x G1, 24x G2, 8x G3) and normal (2x)/hyperplastic (11x) oral mucosa was subjected to laminin chain and alpha-smooth muscle actin (ASMA) immunohistochemistry. Results were correlated to tumour grade. The relation of laminin chain positive vessels to total vessel number was assessed by immunofluorescence double labelling with CD31. Results: Stromal laminin alpha2 chain significantly decreases and alpha3, alpha4, alpha5 and gamma2 chains and also ASMA significantly increase with rising grade. The amount of stromal alpha3, alpha4 and gamma2 chains significantly increased with rising ASMA positivity. There is a significant decrease in alpha3 chain positive vessels with neoplastic transformation. Conclusions: Mediated by myofibroblasts, OSCC development is associated with a stromal up-regulation of laminin isoforms possibly contributing to a migration promoting microenvironment. A vascular basement membrane reorganization concerning alpha3 and gamma2 chain laminins during tumour angioneogenesis is suggested. J Oral Pathol Med (2009).}, author = {Franz, Marcus and Wolheim, Anke and Richter, Petra and Umbreit, Claudia and Dahse, Regine and Driemel, Oliver and Hyckel, Peter and Virtanen, Ismo and Kosmehl, Hartwig and Berndt, Alexander}, citeulike-article-id = {6097087}, citeulike-linkout-0 = {http://dx.doi.org/10.1111/j.1600-0714.2009.00840.x}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19889153}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19889153}, day = {2}, doi = {10.1111/j.1600-0714.2009.00840.x}, issn = {1600-0714}, journal = {Journal of oral pathology \& medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology}, month = {November}, posted-at = {2009-11-11 08:15:56}, priority = {2}, title = {Stromal laminin chain distribution in normal, hyperplastic and malignant oral mucosa: relation to myofibroblast occurrence and vessel formation.}, url = {http://dx.doi.org/10.1111/j.1600-0714.2009.00840.x}, year = {2009} }

TY - JOUR ID - citeulike:6097087 L3 - citeulike-article-id:6097087 N2 - Background: The contribution of stromal laminin chain expression to malignant potential, tumour stroma reorganization and vessel formation in oral squamous cell carcinoma (OSCC) is not fully understood. Therefore, the expression of the laminin chains alpha2, alpha3, alpha4, alpha5 and gamma2 in the stromal compartment/vascular structures in OSCC was analysed. Methods: Frozen tissue of OSCC (9x G1, 24x G2, 8x G3) and normal (2x)/hyperplastic (11x) oral mucosa was subjected to laminin chain and alpha-smooth muscle actin (ASMA) immunohistochemistry. Results were correlated to tumour grade. The relation of laminin chain positive vessels to total vessel number was assessed by immunofluorescence double labelling with CD31. Results: Stromal laminin alpha2 chain significantly decreases and alpha3, alpha4, alpha5 and gamma2 chains and also ASMA significantly increase with rising grade. The amount of stromal alpha3, alpha4 and gamma2 chains significantly increased with rising ASMA positivity. There is a significant decrease in alpha3 chain positive vessels with neoplastic transformation. Conclusions: Mediated by myofibroblasts, OSCC development is associated with a stromal up-regulation of laminin isoforms possibly contributing to a migration promoting microenvironment. A vascular basement membrane reorganization concerning alpha3 and gamma2 chain laminins during tumour angioneogenesis is suggested. J Oral Pathol Med (2009). JF - Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology SN - 1600-0714 TI - Stromal laminin chain distribution in normal, hyperplastic and malignant oral mucosa: relation to myofibroblast occurrence and vessel formation. AU - Franz, Marcus AU - Wolheim, Anke AU - Richter, Petra AU - Umbreit, Claudia AU - Dahse, Regine AU - Driemel, Oliver AU - Hyckel, Peter AU - Virtanen, Ismo AU - Kosmehl, Hartwig AU - Berndt, Alexander PY - 2009/11/02/ UR - http://dx.doi.org/10.1111/j.1600-0714.2009.00840.x ER -