Simple analytic method of 11C-flumazenil metabolite in blood. Export

@article{citeulike:2619075, abstract = {11C-Flumazenil ((11)C-FMZ) is useful to estimate central benzodiazepine receptors by PET. The binding potential (BP) can be calculated with dynamic PET and continual blood sampling. Because conventional metabolite analysis of plasma samples is complicated and time consuming, a simple method is required to obtain an input function. In this article, a whole blood solvent extraction method was evaluated using data of 13 subjects. METHODS: The plasma solvent extraction method was estimated in comparison with the thin-layer chromatography (TLC) method. Then, the whole blood solvent extraction method was evaluated in comparison with the plasma solvent extraction method. RESULTS: Metabolite data analyzed by the plasma extraction method were well correlated with those by the TLC method (r = 0.99). The BP was calculated using both the whole blood extraction data and the plasma extraction data. No difference was observed in all brain regions. CONCLUSION: This simple whole blood solvent extraction method can be applied to clinical BP estimation using (11)C-FMZ.}, address = {Photon Medical Research Center, Hamamatsu University School of Medicine, Handayama, Hamamatsu, Japan. magata@hama-med.ac.jp}, author = {Magata, Y. and Mukai, T. and Ihara, M. and Nishizawa, S. and Kitano, H. and Ishizu, K. and Saji, H. and Konishi, J.}, citeulike-article-id = {2619075}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/12621009}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=12621009}, issn = {0161-5505}, journal = {J Nucl Med}, keywords = {flumazenil, human, kinetic\_modeling}, month = {March}, number = {3}, pages = {417--421}, posted-at = {2008-04-01 10:39:53}, priority = {2}, title = {Simple analytic method of 11C-flumazenil metabolite in blood.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/12621009}, volume = {44}, year = {2003} }

TY - JOUR ID - citeulike:2619075 L3 - citeulike-article-id:2619075 N2 - 11C-Flumazenil ((11)C-FMZ) is useful to estimate central benzodiazepine receptors by PET. The binding potential (BP) can be calculated with dynamic PET and continual blood sampling. Because conventional metabolite analysis of plasma samples is complicated and time consuming, a simple method is required to obtain an input function. In this article, a whole blood solvent extraction method was evaluated using data of 13 subjects. METHODS: The plasma solvent extraction method was estimated in comparison with the thin-layer chromatography (TLC) method. Then, the whole blood solvent extraction method was evaluated in comparison with the plasma solvent extraction method. RESULTS: Metabolite data analyzed by the plasma extraction method were well correlated with those by the TLC method (r = 0.99). The BP was calculated using both the whole blood extraction data and the plasma extraction data. No difference was observed in all brain regions. CONCLUSION: This simple whole blood solvent extraction method can be applied to clinical BP estimation using (11)C-FMZ. IS - 3 JF - J Nucl Med SN - 0161-5505 AD - Photon Medical Research Center, Hamamatsu University School of Medicine, Handayama, Hamamatsu, Japan. magata@hama-med.ac.jp EP - 421 TI - Simple analytic method of 11C-flumazenil metabolite in blood. VL - 44 SP - 417 KW - flumazenil KW - human KW - kinetic_modeling AU - Magata, Y AU - Mukai, T AU - Ihara, M AU - Nishizawa, S AU - Kitano, H AU - Ishizu, K AU - Saji, H AU - Konishi, J PY - 2003/03// UR - http://view.ncbi.nlm.nih.gov/pubmed/12621009 ER -