Validation of a rapid equilibrium dialysis approach for the measurement of plasma protein binding. Export

@article{citeulike:2737641, abstract = {Equilibrium dialysis (ED) is one of the most frequently used approaches to investigate drug binding, where the major drawbacks are the time to reach equilibrium (varying between 6 and 24 h), a long assay preparation time and complexity of automation. A rapid equilibrium dialysis (RED) device has recently become commercially available (Pierce Biotechnology, ThermoFisher Scientific, Waltham, MA) offering the potential for reduced preparation and equilibration times. The RED device comprises a Teflon base plate which holds up to 48 disposable dialysis cells. Each dialysis insert is made up of two side-by-side chambers separated by a vertical cylinder of dialysis membrane with a high membrane surface area-to-volume ratio. An independent validation of the RED approach for the measurement of human plasma protein binding (PPB) was carried out as a comparative analysis with standard ED evaluating equilibration time, assay reproducibility and accuracy and ease of use. Using a diverse set of 18 commercially available drugs spanning a range of physicochemical properties we have shown this to be a robust and accurate methodology, with a shorter preparation and dialysis time, capable of being automated as a high-throughput assay for the determination of PPB. (c) 2008 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci.}, address = {Metabolism and Pharmacokinetics Group, Novartis Institutes for Biomedical Research, Wimblehurst Road, Horsham, West Sussex RH12 5AB, UK.}, author = {Waters, Nigel J J. and Jones, Rachel and Williams, Gareth and Sohal, Bindi}, citeulike-article-id = {2737641}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/jps.21317}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18300299}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18300299}, day = {25}, doi = {10.1002/jps.21317}, issn = {0022-3549}, journal = {Journal of pharmaceutical sciences}, keywords = {dialysis, kinetic\_modeling}, month = {February}, posted-at = {2008-04-30 13:02:40}, priority = {3}, title = {Validation of a rapid equilibrium dialysis approach for the measurement of plasma protein binding.}, url = {http://dx.doi.org/10.1002/jps.21317}, year = {2008} }

TY - JOUR ID - citeulike:2737641 L3 - citeulike-article-id:2737641 N2 - Equilibrium dialysis (ED) is one of the most frequently used approaches to investigate drug binding, where the major drawbacks are the time to reach equilibrium (varying between 6 and 24 h), a long assay preparation time and complexity of automation. A rapid equilibrium dialysis (RED) device has recently become commercially available (Pierce Biotechnology, ThermoFisher Scientific, Waltham, MA) offering the potential for reduced preparation and equilibration times. The RED device comprises a Teflon base plate which holds up to 48 disposable dialysis cells. Each dialysis insert is made up of two side-by-side chambers separated by a vertical cylinder of dialysis membrane with a high membrane surface area-to-volume ratio. An independent validation of the RED approach for the measurement of human plasma protein binding (PPB) was carried out as a comparative analysis with standard ED evaluating equilibration time, assay reproducibility and accuracy and ease of use. Using a diverse set of 18 commercially available drugs spanning a range of physicochemical properties we have shown this to be a robust and accurate methodology, with a shorter preparation and dialysis time, capable of being automated as a high-throughput assay for the determination of PPB. (c) 2008 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci. JF - Journal of pharmaceutical sciences SN - 0022-3549 AD - Metabolism and Pharmacokinetics Group, Novartis Institutes for Biomedical Research, Wimblehurst Road, Horsham, West Sussex RH12 5AB, UK. TI - Validation of a rapid equilibrium dialysis approach for the measurement of plasma protein binding. KW - dialysis KW - kinetic_modeling AU - Waters, Nigel J AU - Jones, Rachel AU - Williams, Gareth AU - Sohal, Bindi PY - 2008/02/25/ UR - http://dx.doi.org/10.1002/jps.21317 ER -