Vibrio parahaemolyticus orchestrates a multifaceted host cell infection by induction of autophagy, cell rounding, and then cell lysis Export

@article{citeulike:3190637, abstract = {10.1073/pnas.0802773105 The bacterial pathogen utilizes a type III secretion system to cause death of host cells within hours of infection. We report that cell death is completely independent of apoptosis and occurs by a mechanism in which injection of multiple type III effectors causes induction of autophagy, cell rounding, and the subsequent release of cellular contents. Autophagy is detected by the appearance of lipidated light chain 3 (LC3) and by increases in punctae and vacuole formation. Electron microscopy reveals the production of early autophagic vesicles during infection. Consistent with phosphoinositide 3 (PI3) kinase playing a role in autophagy, treatment of infected cells with a PI3 kinase inhibitor attenuates autophagy in infected cells. Because many effectors are injected during a infection, it is not surprising that the presence of a sole PI3 kinase inhibitor does not prevent inevitable host-cell death. Our studies reveal an infection paradigm whereby an extracellular pathogen uses its type III secretion system to cause at least three parallel events that eventually result in the proinflammatory death of an infected host cell.}, author = {Burdette, Dara L. and Yarbrough, Melanie L. and Orvedahl, Anthony and Gilpin, Christopher J. and Orth, Kim}, citeulike-article-id = {3190637}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0802773105}, citeulike-linkout-1 = {http://www.pnas.org/content/105/34/12497.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/105/34/12497.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/18713860}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=18713860}, day = {26}, doi = {10.1073/pnas.0802773105}, journal = {Proceedings of the National Academy of Sciences}, keywords = {bacteria, cell}, number = {34}, pages = {12497--12502}, posted-at = {2008-09-04 09:58:38}, priority = {2}, title = {Vibrio parahaemolyticus orchestrates a multifaceted host cell infection by induction of autophagy, cell rounding, and then cell lysis}, url = {http://dx.doi.org/10.1073/pnas.0802773105}, volume = {105}, year = {2008} }

TY - JOUR ID - citeulike:3190637 L3 - citeulike-article-id:3190637 N2 - 10.1073/pnas.0802773105 The bacterial pathogen utilizes a type III secretion system to cause death of host cells within hours of infection. We report that cell death is completely independent of apoptosis and occurs by a mechanism in which injection of multiple type III effectors causes induction of autophagy, cell rounding, and the subsequent release of cellular contents. Autophagy is detected by the appearance of lipidated light chain 3 (LC3) and by increases in punctae and vacuole formation. Electron microscopy reveals the production of early autophagic vesicles during infection. Consistent with phosphoinositide 3 (PI3) kinase playing a role in autophagy, treatment of infected cells with a PI3 kinase inhibitor attenuates autophagy in infected cells. Because many effectors are injected during a infection, it is not surprising that the presence of a sole PI3 kinase inhibitor does not prevent inevitable host-cell death. Our studies reveal an infection paradigm whereby an extracellular pathogen uses its type III secretion system to cause at least three parallel events that eventually result in the proinflammatory death of an infected host cell. IS - 34 JF - Proceedings of the National Academy of Sciences EP - 12502 TI - Vibrio parahaemolyticus orchestrates a multifaceted host cell infection by induction of autophagy, cell rounding, and then cell lysis VL - 105 SP - 12497 KW - bacteria KW - cell AU - Burdette, Dara AU - Yarbrough, Melanie AU - Orvedahl, Anthony AU - Gilpin, Christopher AU - Orth, Kim PY - 2008//26/ UR - http://dx.doi.org/10.1073/pnas.0802773105 ER -