The cohesin ring concatenates sister DNA molecules Export

@article{citeulike:2955280, abstract = {Sister chromatid cohesion, which is essential for mitosis, is mediated by a multi-subunit protein complex called cohesin. Cohesin's Scc1, Smc1 and Smc3 subunits form a tripartite ring structure, and it has been proposed that cohesin holds sister DNA molecules together by trapping them inside its ring. To test this, we used site-specific crosslinking to create chemical connections at the three interfaces between the three constituent polypeptides of the ring, thereby creating covalently closed cohesin rings. As predicted by the ring entrapment model, this procedure produced dimeric DNA–cohesin structures that are resistant to protein denaturation. We conclude that cohesin rings concatenate individual sister minichromosome DNA molecules.}, author = {Haering, Christian H. and Farcas, Ana-Maria and Arumugam, Prakash and Metson, Jean and Nasmyth, Kim}, citeulike-article-id = {2955280}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nature07098}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nature07098}, day = {02}, doi = {10.1038/nature07098}, issn = {0028-0836}, journal = {Nature}, keywords = {cohesin, cohesion, crosslinking}, month = {July}, number = {7202}, pages = {297--301}, posted-at = {2009-10-29 13:37:06}, priority = {5}, publisher = {Nature Publishing Group}, title = {The cohesin ring concatenates sister DNA molecules}, url = {http://dx.doi.org/10.1038/nature07098}, volume = {454}, year = {2008} }

TY - JOUR ID - citeulike:2955280 L3 - citeulike-article-id:2955280 N2 - Sister chromatid cohesion, which is essential for mitosis, is mediated by a multi-subunit protein complex called cohesin. Cohesin's Scc1, Smc1 and Smc3 subunits form a tripartite ring structure, and it has been proposed that cohesin holds sister DNA molecules together by trapping them inside its ring. To test this, we used site-specific crosslinking to create chemical connections at the three interfaces between the three constituent polypeptides of the ring, thereby creating covalently closed cohesin rings. As predicted by the ring entrapment model, this procedure produced dimeric DNA–cohesin structures that are resistant to protein denaturation. We conclude that cohesin rings concatenate individual sister minichromosome DNA molecules. IS - 7202 JF - Nature SN - 0028-0836 EP - 301 TI - The cohesin ring concatenates sister DNA molecules PB - Nature Publishing Group VL - 454 SP - 297 KW - cohesin KW - cohesion KW - crosslinking AU - Haering, Christian AU - Farcas, Ana-Maria AU - Arumugam, Prakash AU - Metson, Jean AU - Nasmyth, Kim PY - 2008/07/02/ UR - http://dx.doi.org/10.1038/nature07098 ER -