Interleukin-6 Production by Schwann Cells and Induction in Sciatic Nerve Injury Export

@article{citeulike:2192373, abstract = {Abstract: Interleukin-6 (IL-6) was produced by the spontaneously immortal Schwann cell clone, iSC, when cocultured with PC12 cells. The iSC cell-derived IL-6 in coculture conditioned media caused the neuronal differentiation of naive PC12 cells and this bioactivity was neutralized by preincubation of conditioned media with antisera to IL-6. Cocultured iSC transcribe IL-6 message as confirmed by northern analysis. Stimuli that induce IL-6 production in the hematopoietic lineage induced transcription and production of IL-6 by iSC cells. Lipopolysaccharide, tumor necrosis factor-alpha, IL-1alpha, IL-6, and serum withdrawal induced iSC cell IL-6 mRNA. The kinetics of IL-6 production was confirmed in the mouse IL-6-dependent B9 bioassay and that activity could be neutralized with antisera to IL-6. Expression of both the IL-6 receptor and the gp130 signal transduction component by iSC as determined by northern analysis suggests an autocrine regulatory mechanism. The observed iSC production of IL-6 in vitro led to an investigation of the sciatic nerve crush model of Schwann cell activation in vivo. In the initial 12 h after crush injury, IL-6 message is induced. IL-6 mRNA expression was highest distal to the crush injury. Our in vitro data demonstrate that iSC cells produce IL-6 in response to PC12 cell coculture and to stimuli that induce IL-6 production in the hematopoietic lineage. The induction of IL-6 message distal to a crush injury suggests another mechanism by which Schwann cells facilitate peripheral nerve regeneration.}, author = {Bolin, Laurel M. and Verity, Neil A. and Silver, Jon E. and Shooter, Eric M. and Abrams, John S.}, citeulike-article-id = {2192373}, citeulike-linkout-0 = {http://www.blackwell-synergy.com/doi/abs/10.1046/j.1471-4159.1995.64020850.x}, citeulike-linkout-1 = {http://dx.doi.org/10.1046/j.1471-4159.1995.64020850.x}, doi = {10.1046/j.1471-4159.1995.64020850.x}, journal = {Journal of Neurochemistry}, keywords = {6, il}, number = {2}, pages = {850--858}, posted-at = {2008-01-03 21:45:20}, priority = {2}, title = {Interleukin-6 Production by Schwann Cells and Induction in Sciatic Nerve Injury}, url = {http://dx.doi.org/10.1046/j.1471-4159.1995.64020850.x}, volume = {64}, year = {1995} }

TY - JOUR ID - citeulike:2192373 L3 - citeulike-article-id:2192373 N2 - Abstract: Interleukin-6 (IL-6) was produced by the spontaneously immortal Schwann cell clone, iSC, when cocultured with PC12 cells. The iSC cell-derived IL-6 in coculture conditioned media caused the neuronal differentiation of naive PC12 cells and this bioactivity was neutralized by preincubation of conditioned media with antisera to IL-6. Cocultured iSC transcribe IL-6 message as confirmed by northern analysis. Stimuli that induce IL-6 production in the hematopoietic lineage induced transcription and production of IL-6 by iSC cells. Lipopolysaccharide, tumor necrosis factor-alpha, IL-1alpha, IL-6, and serum withdrawal induced iSC cell IL-6 mRNA. The kinetics of IL-6 production was confirmed in the mouse IL-6-dependent B9 bioassay and that activity could be neutralized with antisera to IL-6. Expression of both the IL-6 receptor and the gp130 signal transduction component by iSC as determined by northern analysis suggests an autocrine regulatory mechanism. The observed iSC production of IL-6 in vitro led to an investigation of the sciatic nerve crush model of Schwann cell activation in vivo. In the initial 12 h after crush injury, IL-6 message is induced. IL-6 mRNA expression was highest distal to the crush injury. Our in vitro data demonstrate that iSC cells produce IL-6 in response to PC12 cell coculture and to stimuli that induce IL-6 production in the hematopoietic lineage. The induction of IL-6 message distal to a crush injury suggests another mechanism by which Schwann cells facilitate peripheral nerve regeneration. IS - 2 JF - Journal of Neurochemistry EP - 858 TI - Interleukin-6 Production by Schwann Cells and Induction in Sciatic Nerve Injury VL - 64 SP - 850 KW - 6 KW - il AU - Bolin, Laurel AU - Verity, Neil AU - Silver, Jon AU - Shooter, Eric AU - Abrams, John PY - 1995/// UR - http://dx.doi.org/10.1046/j.1471-4159.1995.64020850.x ER -