Coordinate regulation of PSP/reg and PAP isoforms as a family of secretory stress proteins in an animal model of chronic pancreatitis Export

@article{Bimmler2004, abstract = {BackgroundPSP/reg and PAP are secretory stress proteins (SSP) and may be part of a protective mechanism. They share structural homologies and form insoluble fibrils after tryptic activation. To further explore the regulation of these proteins, we investigated the male WBN/Kob rat, a model of pancreatic inflammatory and fibrotic disease similar to chronic pancreatitis.Materials and methodsExpression of PSP/reg and PAP I, II and III in the WBN/Kob rat pancreas was evaluated on the mRNA and protein level, by immunohistochemistry and by highly sensitive isoform specific ELISAs.ResultsThe SSPs are constitutively secreted, PAP in nanomolar, PSP/reg in micromolar concentrations. Before conventional morphological changes are detectable in the WBN/Kob rat, focally increased expression of secretory stress protein is visible. SSP levels in pancreatic juice of WBN/Kob rats reach peak values 10- to 50-fold higher than in Wistar control rats. The highest expression was localized to acini with inflammatory infiltration.ConclusionsThere is a tight spatial and temporal association between pre-inflammatory changes or inflammation and SSP-expression. These results support our concept that PSP/reg and PAP are coordinately regulated SSP.}, author = {Bimmler, Daniel and Schiesser, Marc and Perren, Aurel and Scheele, George and Angst, Eliane and Meili, Severin and Ammann, Rudolf and Graf, Rolf}, citeulike-article-id = {780889}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/S0022-4804(03)00342-1}, citeulike-linkout-1 = {http://www.sciencedirect.com/science/article/B6WM6-4C7W8HT-4/2/dad52d8b6282c0b5a28406ec482fccd3}, day = {15}, doi = {10.1016/S0022-4804(03)00342-1}, journal = {Journal of Surgical Research}, keywords = {methods, mice, pancreatitis, pap, regulation}, month = {May}, number = {2}, pages = {122--135}, posted-at = {2006-07-31 11:18:13}, priority = {2}, title = {Coordinate regulation of PSP/reg and PAP isoforms as a family of secretory stress proteins in an animal model of chronic pancreatitis}, url = {http://dx.doi.org/10.1016/S0022-4804(03)00342-1}, volume = {118}, year = {2004} }

TY - JOUR ID - Bimmler2004 L3 - citeulike-article-id:780889 N2 - BackgroundPSP/reg and PAP are secretory stress proteins (SSP) and may be part of a protective mechanism. They share structural homologies and form insoluble fibrils after tryptic activation. To further explore the regulation of these proteins, we investigated the male WBN/Kob rat, a model of pancreatic inflammatory and fibrotic disease similar to chronic pancreatitis.Materials and methodsExpression of PSP/reg and PAP I, II and III in the WBN/Kob rat pancreas was evaluated on the mRNA and protein level, by immunohistochemistry and by highly sensitive isoform specific ELISAs.ResultsThe SSPs are constitutively secreted, PAP in nanomolar, PSP/reg in micromolar concentrations. Before conventional morphological changes are detectable in the WBN/Kob rat, focally increased expression of secretory stress protein is visible. SSP levels in pancreatic juice of WBN/Kob rats reach peak values 10- to 50-fold higher than in Wistar control rats. The highest expression was localized to acini with inflammatory infiltration.ConclusionsThere is a tight spatial and temporal association between pre-inflammatory changes or inflammation and SSP-expression. These results support our concept that PSP/reg and PAP are coordinately regulated SSP. IS - 2 JF - Journal of Surgical Research EP - 135 TI - Coordinate regulation of PSP/reg and PAP isoforms as a family of secretory stress proteins in an animal model of chronic pancreatitis VL - 118 SP - 122 KW - methods KW - mice KW - pancreatitis KW - pap KW - regulation AU - Bimmler, Daniel AU - Schiesser, Marc AU - Perren, Aurel AU - Scheele, George AU - Angst, Eliane AU - Meili, Severin AU - Ammann, Rudolf AU - Graf, Rolf PY - 2004/05/15/ UR - http://dx.doi.org/10.1016/S0022-4804(03)00342-1 ER -