Release of interleukin-6 via the regulated secretory pathway in PC12 cells Export

@article{Moller2006, abstract = {A growing body of evidence suggests that diverse growth factors such as neurotrophins (NTs), insulin-like growth factor-1 (IGF-1), and glial cell line-derived neurotrophic factor (GDNF) can be released via the regulated secretory pathway in neuronal cells, possibly representing a mechanism for preferentially supplying these growth factors to active synapses. Here we investigated whether interleukin-6 (IL-6), a member of the family of neuropoietic cytokines, can be released via stimulus-coupled secretion as well. IL-6 was expressed in PC12 cells, a neuronal model cell line that is frequently used for the study of vesicle release and trafficking. Regulated secretion of this cytokine was induced by 0.5 mM ATP and treatment with epidermal growth factor (EGF) and nerve growth factor (NGF). Release induced by 0.5 mM ATP but not by NGF or EGF depended on the presence of extracellular Ca++. Furthermore, IL-6 colocalized with the dense core vesicle (DCV)-marker secretogranin-II (Sg-II) in transfected PC12 cells. Our data suggest that the neuropoietic cytokine IL-6 can be sorted to the regulated secretory pathway in neuronal cells and indicate a potential role for this cytokine in synaptic plasticity.}, author = {Moller, Jens C. and Kruttgen, Alex and Burmester, Rosi and Weis, Joachim and Oertel, Wolfgang H. and Shooter, Eric M.}, citeulike-article-id = {812556}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.neulet.2006.02.018}, citeulike-linkout-1 = {http://www.sciencedirect.com/science/article/B6T0G-4JCBRKG-6/2/46a261b80cad174aba4550cdc364325e}, day = {29}, doi = {10.1016/j.neulet.2006.02.018}, journal = {Neuroscience Letters}, keywords = {function, il6, neuropoetic}, month = {May}, number = {1-2}, pages = {75--79}, posted-at = {2006-08-22 11:54:25}, priority = {2}, title = {Release of interleukin-6 via the regulated secretory pathway in PC12 cells}, url = {http://dx.doi.org/10.1016/j.neulet.2006.02.018}, volume = {400}, year = {2006} }

TY - JOUR ID - Moller2006 L3 - citeulike-article-id:812556 N2 - A growing body of evidence suggests that diverse growth factors such as neurotrophins (NTs), insulin-like growth factor-1 (IGF-1), and glial cell line-derived neurotrophic factor (GDNF) can be released via the regulated secretory pathway in neuronal cells, possibly representing a mechanism for preferentially supplying these growth factors to active synapses. Here we investigated whether interleukin-6 (IL-6), a member of the family of neuropoietic cytokines, can be released via stimulus-coupled secretion as well. IL-6 was expressed in PC12 cells, a neuronal model cell line that is frequently used for the study of vesicle release and trafficking. Regulated secretion of this cytokine was induced by 0.5 mM ATP and treatment with epidermal growth factor (EGF) and nerve growth factor (NGF). Release induced by 0.5 mM ATP but not by NGF or EGF depended on the presence of extracellular Ca++. Furthermore, IL-6 colocalized with the dense core vesicle (DCV)-marker secretogranin-II (Sg-II) in transfected PC12 cells. Our data suggest that the neuropoietic cytokine IL-6 can be sorted to the regulated secretory pathway in neuronal cells and indicate a potential role for this cytokine in synaptic plasticity. IS - 1-2 JF - Neuroscience Letters EP - 79 TI - Release of interleukin-6 via the regulated secretory pathway in PC12 cells VL - 400 SP - 75 KW - function KW - il6 KW - neuropoetic AU - Moller, Jens AU - Kruttgen, Alex AU - Burmester, Rosi AU - Weis, Joachim AU - Oertel, Wolfgang AU - Shooter, Eric PY - 2006/05/29/ UR - http://dx.doi.org/10.1016/j.neulet.2006.02.018 ER -