Dynamic Pattern of Reg-2 Expression in Rat Sensory Neurons after Peripheral Nerve Injury Export

@article{Averill2001, abstract = {The 16 kDa pancreatitis-associated protein Reg-2 has recently been shown to facilitate the regeneration of motor and sensory neurons after peripheral nerve injury in the adult rat. Reg-2 has also been shown to be a neurotrophic factor that is an essential intermediate in the pathways through which CNTF supports the survival of motor neurons during development. Here we report the dynamic expression of Reg-2 in rat sensory neurons after peripheral nerve injury. Reg-2 is normally not expressed by dorsal root ganglion (DRG) cells, but we show, using immunocytochemistry, that Reg-2 is rapidly upregulated in DRG cells after sciatic nerve transection and after 24 hr recovery is expressed almost exclusively in small-diameter neurons that bind the lectin Griffonia simplicifolia IB4 and express the purinoceptor P2X3. However, by 7 d after axotomy, Reg-2 is expressed in medium to large neurons and coexists partly with the neuropeptides galanin and neuropeptide Y, which are also upregulated after peripheral nerve transection. At this time point, Reg-2 is no longer expressed in small neurons, and there is no colocalization with IB4 binding neurons, demonstrating a shift in Reg-2 expression from one subset of DRG neurons to another. We also show by double labeling for activating transcription factor 3, a transcription factor that is upregulated after nerve injury, that Reg-2 expression occurs predominantly in axotomized DRG cells but that a small percentage of uninjured DRG cells also upregulate Reg-2. The selective expression within IB4/P2X3 cells, and the dynamic shift from small to large cells, is unique among DRG peptides and suggests that Reg-2 has a distinctive role in the injury response. 20026667}, author = {Averill, Sharon and Davis, Danny R. and Shortland, Peter J. and Priestley, John V. and Hunt, Stephen P.}, citeulike-article-id = {815406}, citeulike-linkout-0 = {http://www.jneurosci.org/cgi/content/abstract/22/17/7493}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/12196572}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=12196572}, day = {1}, journal = {J. Neurosci.}, keywords = {neuron, pap}, month = {September}, number = {17}, pages = {7493--7501}, posted-at = {2006-08-24 10:49:22}, priority = {2}, title = {Dynamic Pattern of Reg-2 Expression in Rat Sensory Neurons after Peripheral Nerve Injury}, url = {http://www.jneurosci.org/cgi/content/abstract/22/17/7493}, volume = {22}, year = {2002} }

TY - JOUR ID - Averill2001 L3 - citeulike-article-id:815406 N2 - The 16 kDa pancreatitis-associated protein Reg-2 has recently been shown to facilitate the regeneration of motor and sensory neurons after peripheral nerve injury in the adult rat. Reg-2 has also been shown to be a neurotrophic factor that is an essential intermediate in the pathways through which CNTF supports the survival of motor neurons during development. Here we report the dynamic expression of Reg-2 in rat sensory neurons after peripheral nerve injury. Reg-2 is normally not expressed by dorsal root ganglion (DRG) cells, but we show, using immunocytochemistry, that Reg-2 is rapidly upregulated in DRG cells after sciatic nerve transection and after 24 hr recovery is expressed almost exclusively in small-diameter neurons that bind the lectin Griffonia simplicifolia IB4 and express the purinoceptor P2X3. However, by 7 d after axotomy, Reg-2 is expressed in medium to large neurons and coexists partly with the neuropeptides galanin and neuropeptide Y, which are also upregulated after peripheral nerve transection. At this time point, Reg-2 is no longer expressed in small neurons, and there is no colocalization with IB4 binding neurons, demonstrating a shift in Reg-2 expression from one subset of DRG neurons to another. We also show by double labeling for activating transcription factor 3, a transcription factor that is upregulated after nerve injury, that Reg-2 expression occurs predominantly in axotomized DRG cells but that a small percentage of uninjured DRG cells also upregulate Reg-2. The selective expression within IB4/P2X3 cells, and the dynamic shift from small to large cells, is unique among DRG peptides and suggests that Reg-2 has a distinctive role in the injury response. 20026667 IS - 17 JF - J. Neurosci. EP - 7501 TI - Dynamic Pattern of Reg-2 Expression in Rat Sensory Neurons after Peripheral Nerve Injury VL - 22 SP - 7493 KW - neuron KW - pap AU - Averill, Sharon AU - Davis, Danny AU - Shortland, Peter AU - Priestley, John AU - Hunt, Stephen PY - 2002/09/01/ UR - http://www.jneurosci.org/cgi/content/abstract/22/17/7493 ER -