Refolding, purification, and characterization of human and murine RegIII proteins expressed in Escherichia coli Export

@article{Cash2006, abstract = {The regenerating (Reg) family comprises an extensive, diversified group of proteins with homology to C-type lectins. Several members of this family are highly expressed in the gastrointestinal tract under normal conditions, and often show increased expression in inflammatory bowel disease. However, little is known about Reg protein function, and the carbohydrate ligands for these proteins are poorly characterized. We report here the first expression and purification of Reg proteins using a bacterial system. Mouse RegIII[gamma] and its human counterpart, HIP/PAP, were expressed in Escherichia coli, resulting in the accumulation of aggregated recombinant protein. Both proteins were renatured by arginine-assisted procedures and were further purified using cation-exchange chromatography. The identities of the purified proteins were confirmed by SDS-PAGE, N-terminal sequencing, and MALDI-TOF mass spectrometry. Size exclusion chromatography revealed that both proteins exist as monomers, and circular dichroism showed that their secondary structures exhibit a predominance of [beta]-strands which is typical of C-type lectins. Finally, both RegIII[gamma] and human HIP/PAP bind to mannan but not to monomeric mannose, giving initial insights into their carbohydrate ligands. These studies thus provide an essential foundation for further analyses of human and mouse RegIII protein function.}, author = {Cash, Heather L. and Whitham, Cecilia V. and Hooper, Lora V.}, citeulike-article-id = {842863}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.pep.2006.01.014}, citeulike-linkout-1 = {http://www.sciencedirect.com/science/article/B6WPJ-4J85PJ8-1/2/56fe11a996b5facff04b74c9bb84cc5f}, doi = {10.1016/j.pep.2006.01.014}, journal = {Protein Expression and Purification}, keywords = {3, reg}, month = {July}, number = {1}, pages = {151--159}, posted-at = {2006-09-14 08:05:38}, priority = {2}, title = {Refolding, purification, and characterization of human and murine RegIII proteins expressed in Escherichia coli}, url = {http://dx.doi.org/10.1016/j.pep.2006.01.014}, volume = {48}, year = {2006} }

TY - JOUR ID - Cash2006 L3 - citeulike-article-id:842863 N2 - The regenerating (Reg) family comprises an extensive, diversified group of proteins with homology to C-type lectins. Several members of this family are highly expressed in the gastrointestinal tract under normal conditions, and often show increased expression in inflammatory bowel disease. However, little is known about Reg protein function, and the carbohydrate ligands for these proteins are poorly characterized. We report here the first expression and purification of Reg proteins using a bacterial system. Mouse RegIII[gamma] and its human counterpart, HIP/PAP, were expressed in Escherichia coli, resulting in the accumulation of aggregated recombinant protein. Both proteins were renatured by arginine-assisted procedures and were further purified using cation-exchange chromatography. The identities of the purified proteins were confirmed by SDS-PAGE, N-terminal sequencing, and MALDI-TOF mass spectrometry. Size exclusion chromatography revealed that both proteins exist as monomers, and circular dichroism showed that their secondary structures exhibit a predominance of [beta]-strands which is typical of C-type lectins. Finally, both RegIII[gamma] and human HIP/PAP bind to mannan but not to monomeric mannose, giving initial insights into their carbohydrate ligands. These studies thus provide an essential foundation for further analyses of human and mouse RegIII protein function. IS - 1 JF - Protein Expression and Purification EP - 159 TI - Refolding, purification, and characterization of human and murine RegIII proteins expressed in Escherichia coli VL - 48 SP - 151 KW - 3 KW - reg AU - Cash, Heather AU - Whitham, Cecilia AU - Hooper, Lora PY - 2006/07// UR - http://dx.doi.org/10.1016/j.pep.2006.01.014 ER -