Mouse protein arrays from a TH1 cell cDNA library for antibody screening and serum profiling Export

@article{citeulike:6074549, abstract = {The mouse is the premier genetic model organism for the study of disease and development. We describe the establishment of a mouse T helper cell type 1 (T H 1) protein expression library that provides direct access to thousands of recombinant mouse proteins, in particular those associated with immune responses. The advantage of a system based on the combination of large cDNA expression libraries with microarray technology is the direct connection of the DNA sequence information from a particular clone to its recombinant, expressed protein. We have generated a mouse T H 1 expression cDNA library and used protein arrays of this library to characterize the specificity and cross-reactivity of antibodies. Additionally, we have profiled the autoantibody repertoire in serum of a mouse model for systemic lupus erythematosus on these protein arrays and validated the putative autoantigens on highly sensitive protein microarrays.}, author = {Gutjahr, Claudia and Murphy, Derek and Lueking, Angelika and Koenig, Andrea and Janitz, Michal and O'Brien, John and Korn, Bernhard and Horn, Sabine and Lehrach, Hans and Cahill, Dolores J.}, citeulike-article-id = {6074549}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.ygeno.2004.11.005}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0888754304003106}, doi = {10.1016/j.ygeno.2004.11.005}, issn = {08887543}, journal = {Genomics}, keywords = {antibody, microarray, peptide}, month = {March}, number = {3}, pages = {285--296}, posted-at = {2009-11-05 14:12:26}, priority = {2}, title = {Mouse protein arrays from a TH1 cell cDNA library for antibody screening and serum profiling}, url = {http://dx.doi.org/10.1016/j.ygeno.2004.11.005}, volume = {85}, year = {2005} }

TY - JOUR ID - citeulike:6074549 L3 - citeulike-article-id:6074549 N2 - The mouse is the premier genetic model organism for the study of disease and development. We describe the establishment of a mouse T helper cell type 1 (T H 1) protein expression library that provides direct access to thousands of recombinant mouse proteins, in particular those associated with immune responses. The advantage of a system based on the combination of large cDNA expression libraries with microarray technology is the direct connection of the DNA sequence information from a particular clone to its recombinant, expressed protein. We have generated a mouse T H 1 expression cDNA library and used protein arrays of this library to characterize the specificity and cross-reactivity of antibodies. Additionally, we have profiled the autoantibody repertoire in serum of a mouse model for systemic lupus erythematosus on these protein arrays and validated the putative autoantigens on highly sensitive protein microarrays. IS - 3 JF - Genomics SN - 08887543 EP - 296 TI - Mouse protein arrays from a TH1 cell cDNA library for antibody screening and serum profiling VL - 85 SP - 285 KW - antibody KW - microarray KW - peptide AU - Gutjahr, Claudia AU - Murphy, Derek AU - Lueking, Angelika AU - Koenig, Andrea AU - Janitz, Michal AU - O'Brien, John AU - Korn, Bernhard AU - Horn, Sabine AU - Lehrach, Hans AU - Cahill, Dolores PY - 2005/03// UR - http://dx.doi.org/10.1016/j.ygeno.2004.11.005 ER -