Microarray Profiling of Antibody Responses against Simian-Human Immunodeficiency Virus: Postchallenge Convergence of Reactivities Independent of Host Histocompatibility Type and Vaccine Regimen Export

@article{citeulike:6074582, abstract = {We developed antigen microarrays to profile the breadth, strength, and kinetics of epitope-specific antiviral antibody responses in vaccine trials with a simian-human immunodeficiency virus (SHIV) model for human immunodeficiency virus (HIV) infection. These arrays contained 430 distinct proteins and overlapping peptides spanning the SHIV proteome. In macaques vaccinated with three different DNA and/or recombinant modified vaccinia virus Ankara (rMVA) vaccines encoding Gag-Pol or Gag-Pol-Env, these arrays distinguished vaccinated from challenged macaques, identified three novel viral epitopes, and predicted survival. Following viral challenge, anti-SHIV antibody responses ultimately converged to target eight immunodominant B-cell regions in Env regardless of vaccine regimen, host histocompatibility type, and divergent T-cell specificities. After challenge, responses to nonimmunodominant epitopes were transient, while responses to dominant epitopes were gained. These data suggest that the functional diversity of anti-SHIV B-cell responses is highly limited in the presence of persisting antigen. 10.1128/JVI.77.20.11125-11138.2003}, author = {Neuman de Vegvar, Henry E. and Amara, Rama R. and Steinman, Lawrence and Utz, Paul J. and Robinson, Harriet L. and Robinson, William H.}, citeulike-article-id = {6074582}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/JVI.77.20.11125-11138.2003}, citeulike-linkout-1 = {http://jvi.asm.org/content/77/20/11125.abstract}, citeulike-linkout-2 = {http://jvi.asm.org/content/77/20/11125.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/14512560}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=14512560}, day = {15}, doi = {10.1128/JVI.77.20.11125-11138.2003}, journal = {J. Virol.}, keywords = {antibody, immunology, microarray, peptide, siv}, month = {October}, number = {20}, pages = {11125--11138}, posted-at = {2009-11-05 14:22:22}, priority = {2}, title = {Microarray Profiling of Antibody Responses against Simian-Human Immunodeficiency Virus: Postchallenge Convergence of Reactivities Independent of Host Histocompatibility Type and Vaccine Regimen}, url = {http://dx.doi.org/10.1128/JVI.77.20.11125-11138.2003}, volume = {77}, year = {2003} }

TY - JOUR ID - citeulike:6074582 L3 - citeulike-article-id:6074582 N2 - We developed antigen microarrays to profile the breadth, strength, and kinetics of epitope-specific antiviral antibody responses in vaccine trials with a simian-human immunodeficiency virus (SHIV) model for human immunodeficiency virus (HIV) infection. These arrays contained 430 distinct proteins and overlapping peptides spanning the SHIV proteome. In macaques vaccinated with three different DNA and/or recombinant modified vaccinia virus Ankara (rMVA) vaccines encoding Gag-Pol or Gag-Pol-Env, these arrays distinguished vaccinated from challenged macaques, identified three novel viral epitopes, and predicted survival. Following viral challenge, anti-SHIV antibody responses ultimately converged to target eight immunodominant B-cell regions in Env regardless of vaccine regimen, host histocompatibility type, and divergent T-cell specificities. After challenge, responses to nonimmunodominant epitopes were transient, while responses to dominant epitopes were gained. These data suggest that the functional diversity of anti-SHIV B-cell responses is highly limited in the presence of persisting antigen. 10.1128/JVI.77.20.11125-11138.2003 IS - 20 JF - J. Virol. EP - 11138 TI - Microarray Profiling of Antibody Responses against Simian-Human Immunodeficiency Virus: Postchallenge Convergence of Reactivities Independent of Host Histocompatibility Type and Vaccine Regimen VL - 77 SP - 11125 KW - antibody KW - immunology KW - microarray KW - peptide KW - siv AU - Neuman de Vegvar, Henry AU - Amara, Rama AU - Steinman, Lawrence AU - Utz, Paul AU - Robinson, Harriet AU - Robinson, William PY - 2003/10/15/ UR - http://dx.doi.org/10.1128/JVI.77.20.11125-11138.2003 ER -