A multiplexed and miniaturized serological tuberculosis assay identifies antigens that discriminate maximally between TB and non-TB sera Export

@article{citeulike:6074583, abstract = {We have developed a multiplexed and miniaturized TB serological assay with the aim of identifying (combinations of) antigens that maximally discriminate between TB and non-TB patients. It features a microarray accommodating 54 TB antigens, less than 1 μl serum consumption and an indirect immunofluorescence detection protocol. With a panel of 20 TB and 80 non-TB sera we ranked combinations of TB antigens with respect to sensitivity and specificity of TB detection by means of logistic step-forward regression analysis. The highest-ranking TB antigen combination had an area-under-the-curve of the receiver–operator-characteristics (ROC) of 0.95. We also identified an antigen that on its own provided good specificity and sensitivity of TB detection (Ara 6 –BSA; area-under-the-ROC curve: 0.90). These area-under-the-ROC curve values are exceptionally high for a serological TB assay. We conclude that TB antigen microarrays permit rapid identification of TB antigens that, either alone or in combination, discriminate maximally between TB and non-TB patients and that such identification provides an excellent starting point for developing point-of-care diagnostic assays.}, author = {Tong, M. and Jacobi, C. and Vanderijke, F. and Kuijper, S. and Vandewerken, S. and Lowary, T. and Hokke, C. and Appelmelk, B. and Nagelkerke, N. and Tanke, H.}, citeulike-article-id = {6074583}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.jim.2005.04.004}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0022175905001110}, doi = {10.1016/j.jim.2005.04.004}, issn = {00221759}, journal = {Journal of Immunological Methods}, keywords = {antibody, antigen, microarray, pathogen, tb}, month = {June}, number = {1-2}, pages = {154--163}, posted-at = {2009-11-05 14:22:47}, priority = {2}, title = {A multiplexed and miniaturized serological tuberculosis assay identifies antigens that discriminate maximally between TB and non-TB sera}, url = {http://dx.doi.org/10.1016/j.jim.2005.04.004}, volume = {301}, year = {2005} }

TY - JOUR ID - citeulike:6074583 L3 - citeulike-article-id:6074583 N2 - We have developed a multiplexed and miniaturized TB serological assay with the aim of identifying (combinations of) antigens that maximally discriminate between TB and non-TB patients. It features a microarray accommodating 54 TB antigens, less than 1 μl serum consumption and an indirect immunofluorescence detection protocol. With a panel of 20 TB and 80 non-TB sera we ranked combinations of TB antigens with respect to sensitivity and specificity of TB detection by means of logistic step-forward regression analysis. The highest-ranking TB antigen combination had an area-under-the-curve of the receiver–operator-characteristics (ROC) of 0.95. We also identified an antigen that on its own provided good specificity and sensitivity of TB detection (Ara 6 –BSA; area-under-the-ROC curve: 0.90). These area-under-the-ROC curve values are exceptionally high for a serological TB assay. We conclude that TB antigen microarrays permit rapid identification of TB antigens that, either alone or in combination, discriminate maximally between TB and non-TB patients and that such identification provides an excellent starting point for developing point-of-care diagnostic assays. IS - 1-2 JF - Journal of Immunological Methods SN - 00221759 EP - 163 TI - A multiplexed and miniaturized serological tuberculosis assay identifies antigens that discriminate maximally between TB and non-TB sera VL - 301 SP - 154 KW - antibody KW - antigen KW - microarray KW - pathogen KW - tb AU - Tong, M AU - Jacobi, C AU - Vanderijke, F AU - Kuijper, S AU - Vandewerken, S AU - Lowary, T AU - Hokke, C AU - Appelmelk, B AU - Nagelkerke, N AU - Tanke, H PY - 2005/06// UR - http://dx.doi.org/10.1016/j.jim.2005.04.004 ER -