High-throughput cloning and expression in recalcitrant bacteria Export

@article{Geertsma2007, abstract = {We developed a generic method for high-throughput cloning in bacteria that are less amenable to conventional DNA manipulations. The method involves ligation-independent cloning in an intermediary Escherichia coli vector, which is rapidly converted via vector-backbone exchange (VBEx) into an organism-specific plasmid ready for high-efficiency transformation. We demonstrated VBEx proof of principle for Lactococcus lactis, but the method can be adapted to all organisms for which plasmids are available.}, author = {Geertsma, Eric R. and Poolman, Bert}, booktitle = {Nat Meth}, citeulike-article-id = {1610924}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nmeth1073}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nmeth1073}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17643108}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17643108}, day = {22}, doi = {10.1038/nmeth1073}, journal = {Nature Methods}, keywords = {dnaassembly, dnasynthesis, homologyrecombination, ligation\_independent\_cloning, syntheticbiology}, month = {July}, number = {9}, pages = {705--707}, posted-at = {2008-11-11 16:55:25}, priority = {3}, publisher = {Nature Publishing Group}, title = {High-throughput cloning and expression in recalcitrant bacteria}, url = {http://dx.doi.org/10.1038/nmeth1073}, volume = {4}, year = {2007} }

TY - JOUR ID - Geertsma2007 L3 - citeulike-article-id:1610924 N2 - We developed a generic method for high-throughput cloning in bacteria that are less amenable to conventional DNA manipulations. The method involves ligation-independent cloning in an intermediary Escherichia coli vector, which is rapidly converted via vector-backbone exchange (VBEx) into an organism-specific plasmid ready for high-efficiency transformation. We demonstrated VBEx proof of principle for Lactococcus lactis, but the method can be adapted to all organisms for which plasmids are available. IS - 9 JF - Nature Methods EP - 707 TI - High-throughput cloning and expression in recalcitrant bacteria PB - Nature Publishing Group VL - 4 T2 - Nat Meth SP - 705 KW - dnaassembly KW - dnasynthesis KW - homologyrecombination KW - ligation_independent_cloning KW - syntheticbiology AU - Geertsma, Eric AU - Poolman, Bert PY - 2007/07/22/ UR - http://dx.doi.org/10.1038/nmeth1073 ER -