Seamless cloning and domain swapping of synthetic and complex DNA. Export

@article{Fernandes2008, abstract = {The use of synthetic DNA can avoid problems that are sometimes encountered with conventional molecular biology techniques using DNA with high GC content, strong secondary structures, or repeat sequences. However, very complex DNA may still resist PCR and synthesis of DNA from oligonucleotides. In the method described here, separately synthesized DNA segments were seamlessly joined independently of the presence of restriction sites in the target DNA. This method allowed the reconstruction of complex DNA by concatenation of easily synthesized segments and permitted repeated swapping of segments, from a few nucleotides to large fragments of complex DNA for phenotypic analysis.}, author = {Fernandes, S. and Tijssen, P.}, citeulike-article-id = {3504077}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.ab.2008.10.008}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0003269708006866}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18976627}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18976627}, day = {1}, doi = {10.1016/j.ab.2008.10.008}, issn = {1096-0309}, journal = {Analytical biochemistry}, keywords = {dnaassembly, dnasynthesis, labprotocol, syntheticbiology}, month = {February}, number = {1}, pages = {171--173}, posted-at = {2008-11-11 16:50:33}, priority = {3}, title = {Seamless cloning and domain swapping of synthetic and complex DNA.}, url = {http://dx.doi.org/10.1016/j.ab.2008.10.008}, volume = {385}, year = {2009} }

TY - JOUR ID - Fernandes2008 L3 - citeulike-article-id:3504077 N2 - The use of synthetic DNA can avoid problems that are sometimes encountered with conventional molecular biology techniques using DNA with high GC content, strong secondary structures, or repeat sequences. However, very complex DNA may still resist PCR and synthesis of DNA from oligonucleotides. In the method described here, separately synthesized DNA segments were seamlessly joined independently of the presence of restriction sites in the target DNA. This method allowed the reconstruction of complex DNA by concatenation of easily synthesized segments and permitted repeated swapping of segments, from a few nucleotides to large fragments of complex DNA for phenotypic analysis. IS - 1 JF - Analytical biochemistry SN - 1096-0309 EP - 173 TI - Seamless cloning and domain swapping of synthetic and complex DNA. VL - 385 SP - 171 KW - dnaassembly KW - dnasynthesis KW - labprotocol KW - syntheticbiology AU - Fernandes, S AU - Tijssen, P PY - 2009/02/01/ UR - http://dx.doi.org/10.1016/j.ab.2008.10.008 ER -