Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels Export

@article{citeulike:2193332, abstract = {Global energy and environmental problems have stimulated increased efforts towards synthesizing biofuels from renewable resources1, 2, 3. Compared to the traditional biofuel, ethanol, higher alcohols offer advantages as gasoline substitutes because of their higher energy density and lower hygroscopicity. In addition, branched-chain alcohols have higher octane numbers compared with their straight-chain counterparts. However, these alcohols cannot be synthesized economically using native organisms. Here we present a metabolic engineering approach using Escherichia coli to produce higher alcohols including isobutanol, 1-butanol, 2-methyl-1-butanol, 3-methyl-1-butanol and 2-phenylethanol from glucose, a renewable carbon source. This strategy uses the host's highly active amino acid biosynthetic pathway and diverts its 2-keto acid intermediates for alcohol synthesis. In particular, we have achieved high-yield, high-specificity production of isobutanol from glucose. The strategy enables the exploration of biofuels beyond those naturally accumulated to high quantities in microbial fermentation.}, author = {Atsumi, Shota and Hanai, Taizo and Liao, James C.}, citeulike-article-id = {2193332}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nature06450}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nature06450}, day = {03}, doi = {10.1038/nature06450}, issn = {0028-0836}, journal = {Nature}, keywords = {alcohols, biofuel, branched-chain, coli, escherichia, higher, isobutanol, non-fermentative, pathways}, month = {January}, number = {7174}, pages = {86--89}, posted-at = {2009-09-17 16:39:58}, priority = {2}, publisher = {Nature Publishing Group}, title = {Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels}, url = {http://dx.doi.org/10.1038/nature06450}, volume = {451}, year = {2008} }

TY - JOUR ID - citeulike:2193332 L3 - citeulike-article-id:2193332 N2 - Global energy and environmental problems have stimulated increased efforts towards synthesizing biofuels from renewable resources1, 2, 3. Compared to the traditional biofuel, ethanol, higher alcohols offer advantages as gasoline substitutes because of their higher energy density and lower hygroscopicity. In addition, branched-chain alcohols have higher octane numbers compared with their straight-chain counterparts. However, these alcohols cannot be synthesized economically using native organisms. Here we present a metabolic engineering approach using Escherichia coli to produce higher alcohols including isobutanol, 1-butanol, 2-methyl-1-butanol, 3-methyl-1-butanol and 2-phenylethanol from glucose, a renewable carbon source. This strategy uses the host's highly active amino acid biosynthetic pathway and diverts its 2-keto acid intermediates for alcohol synthesis. In particular, we have achieved high-yield, high-specificity production of isobutanol from glucose. The strategy enables the exploration of biofuels beyond those naturally accumulated to high quantities in microbial fermentation. IS - 7174 JF - Nature SN - 0028-0836 EP - 89 TI - Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels PB - Nature Publishing Group VL - 451 SP - 86 KW - alcohols KW - biofuel KW - branched-chain KW - coli KW - escherichia KW - higher KW - isobutanol KW - non-fermentative KW - pathways AU - Atsumi, Shota AU - Hanai, Taizo AU - Liao, James PY - 2008/01/03/ UR - http://dx.doi.org/10.1038/nature06450 ER -