Inactivation of the Haemophilus ducreyi luxS Gene Affects the Virulence of This Pathogen in Human Subjects. Export

@article{citeulike:5027737, abstract = {Haemophilus ducreyi 35000HP contains a homologue of the luxS gene, which encodes an enzyme that synthesizes autoinducer 2 (AI-2) in other gram-negative bacteria. H. ducreyi 35000HP produced AI-2 that functioned in a Vibrio harveyi-based reporter system. A H. ducreyi luxS mutant was constructed by insertional inactivation of the luxS gene and lost the ability to produce AI-2. Provision of the H. ducreyi luxS gene in trans partially restored AI-2 production by the mutant. The luxS mutant was compared with its parent for virulence in the human challenge model of experimental chancroid. The pustule-formation rate in 5 volunteers was 93.3\% (95\% confidence interval, 81.7\%-99.9\%) at 15 parent sites and 60.0\% (95\% confidence interval, 48.3\%-71.7\%) at 15 mutant sites (1-tailed [Formula: see text]). Thus, the luxS mutant was partially attenuated for virulence. This is the first report of AI-2 production contributing to the pathogenesis of a genital ulcer disease.}, author = {Labandeira-Rey, Maria and Janowicz, Diane M. and Blick, Robert J. and Fortney, Kate R. and Zwickl, Beth and Katz, Barry P. and Spinola, Stanley M. and Hansen, Eric J.}, citeulike-article-id = {5027737}, citeulike-linkout-0 = {http://dx.doi.org/10.1086/600142}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19552526}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19552526}, citeulike-linkout-3 = {http://www.journals.uchicago.edu/doi/abs/10.1086/600142}, day = {1}, doi = {10.1086/600142}, issn = {0022-1899}, journal = {The Journal of infectious diseases}, keywords = {ducreyi, gene, haemophilus, in, luxs, quorum, sensing, virulence, vivo}, month = {August}, number = {3}, pages = {409--416}, posted-at = {2009-07-03 16:13:15}, priority = {2}, title = {Inactivation of the Haemophilus ducreyi luxS Gene Affects the Virulence of This Pathogen in Human Subjects.}, url = {http://dx.doi.org/10.1086/600142}, volume = {200}, year = {2009} }

TY - JOUR ID - citeulike:5027737 L3 - citeulike-article-id:5027737 N2 - Haemophilus ducreyi 35000HP contains a homologue of the luxS gene, which encodes an enzyme that synthesizes autoinducer 2 (AI-2) in other gram-negative bacteria. H. ducreyi 35000HP produced AI-2 that functioned in a Vibrio harveyi-based reporter system. A H. ducreyi luxS mutant was constructed by insertional inactivation of the luxS gene and lost the ability to produce AI-2. Provision of the H. ducreyi luxS gene in trans partially restored AI-2 production by the mutant. The luxS mutant was compared with its parent for virulence in the human challenge model of experimental chancroid. The pustule-formation rate in 5 volunteers was 93.3% (95% confidence interval, 81.7%-99.9%) at 15 parent sites and 60.0% (95% confidence interval, 48.3%-71.7%) at 15 mutant sites (1-tailed [Formula: see text]). Thus, the luxS mutant was partially attenuated for virulence. This is the first report of AI-2 production contributing to the pathogenesis of a genital ulcer disease. IS - 3 JF - The Journal of infectious diseases SN - 0022-1899 EP - 416 TI - Inactivation of the Haemophilus ducreyi luxS Gene Affects the Virulence of This Pathogen in Human Subjects. VL - 200 SP - 409 KW - ducreyi KW - gene KW - haemophilus KW - in KW - luxs KW - quorum KW - sensing KW - virulence KW - vivo AU - Labandeira-Rey, Maria AU - Janowicz, Diane AU - Blick, Robert AU - Fortney, Kate AU - Zwickl, Beth AU - Katz, Barry AU - Spinola, Stanley AU - Hansen, Eric PY - 2009/08/01/ UR - http://dx.doi.org/10.1086/600142 ER -