Problems with the microbial production of butanol. Export

@article{citeulike:5047656, abstract = {With the incessant fluctuations in oil prices and increasing stress from environmental pollution, renewed attention is being paid to the microbial production of biofuels from renewable sources. As a gasoline substitute, butanol has advantages over traditional fuel ethanol in terms of energy density and hygroscopicity. A variety of cheap substrates have been successfully applied in the production of biobutanol, highlighting the commercial potential of biobutanol development. In this review, in order to better understand the process of acetone-butanol-ethanol production, traditional clostridia fermentation is discussed. Sporulation is probably induced by solvent formation, and the molecular mechanism leading to the initiation of sporulation and solventogenesis is also investigated. Different strategies are employed in the metabolic engineering of clostridia that aim to enhancing solvent production, improve selectivity for butanol production, and increase the tolerance of clostridia to solvents. However, it will be hard to make breakthroughs in the metabolic engineering of clostridia for butanol production without gaining a deeper understanding of the genetic background of clostridia and developing more efficient genetic tools for clostridia. Therefore, increasing attention has been paid to the metabolic engineering of E. coli for butanol production. The importation and expression of a non-clostridial butanol-producing pathway in E. coli is probably the most promising strategy for butanol biosynthesis. Due to the lower butanol titers in the fermentation broth, simultaneous fermentation and product removal techniques have been developed to reduce the cost of butanol recovery. Gas stripping is the best technique for butanol recovery found so far.}, author = {Zheng, Yan-Ning N. and Li, Liang-Zhi Z. and Xian, Mo and Ma, Yu-Jiu J. and Yang, Jian-Ming M. and Xu, Xin and He, Dong-Zhi Z.}, citeulike-article-id = {5047656}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/s10295-009-0609-9}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19562394}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19562394}, citeulike-linkout-3 = {http://www.springerlink.com/content/e6275510513j5448}, doi = {10.1007/s10295-009-0609-9}, issn = {1476-5535}, journal = {Journal of industrial microbiology \& biotechnology}, keywords = {biobutanol, butanol, clostridia, microbial, production, review}, month = {September}, number = {9}, pages = {1127--1138}, posted-at = {2009-07-03 17:09:33}, priority = {2}, title = {Problems with the microbial production of butanol.}, url = {http://dx.doi.org/10.1007/s10295-009-0609-9}, volume = {36}, year = {2009} }

TY - JOUR ID - citeulike:5047656 L3 - citeulike-article-id:5047656 N2 - With the incessant fluctuations in oil prices and increasing stress from environmental pollution, renewed attention is being paid to the microbial production of biofuels from renewable sources. As a gasoline substitute, butanol has advantages over traditional fuel ethanol in terms of energy density and hygroscopicity. A variety of cheap substrates have been successfully applied in the production of biobutanol, highlighting the commercial potential of biobutanol development. In this review, in order to better understand the process of acetone-butanol-ethanol production, traditional clostridia fermentation is discussed. Sporulation is probably induced by solvent formation, and the molecular mechanism leading to the initiation of sporulation and solventogenesis is also investigated. Different strategies are employed in the metabolic engineering of clostridia that aim to enhancing solvent production, improve selectivity for butanol production, and increase the tolerance of clostridia to solvents. However, it will be hard to make breakthroughs in the metabolic engineering of clostridia for butanol production without gaining a deeper understanding of the genetic background of clostridia and developing more efficient genetic tools for clostridia. Therefore, increasing attention has been paid to the metabolic engineering of E. coli for butanol production. The importation and expression of a non-clostridial butanol-producing pathway in E. coli is probably the most promising strategy for butanol biosynthesis. Due to the lower butanol titers in the fermentation broth, simultaneous fermentation and product removal techniques have been developed to reduce the cost of butanol recovery. Gas stripping is the best technique for butanol recovery found so far. IS - 9 JF - Journal of industrial microbiology & biotechnology SN - 1476-5535 EP - 1138 TI - Problems with the microbial production of butanol. VL - 36 SP - 1127 KW - biobutanol KW - butanol KW - clostridia KW - microbial KW - production KW - review AU - Zheng, Yan-Ning AU - Li, Liang-Zhi AU - Xian, Mo AU - Ma, Yu-Jiu AU - Yang, Jian-Ming AU - Xu, Xin AU - He, Dong-Zhi PY - 2009/09// UR - http://dx.doi.org/10.1007/s10295-009-0609-9 ER -